Deregulation of selected microRNAs in sinonasal squamous cell Carcinoma: Searching for Potential Prognostic Biomarkers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F19%3A10399496" target="_blank" >RIV/00064165:_____/19:10399496 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10399496 RIV/00216208:11150/19:10399496 RIV/61989592:15110/19:73595699 RIV/00179906:_____/19:10399496

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=C0HdJdP-dk" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=C0HdJdP-dk</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Deregulation of selected microRNAs in sinonasal squamous cell Carcinoma: Searching for Potential Prognostic Biomarkers

Popis výsledku v původním jazyce

Sinonasal carcinomas are head and neck tumors arising from nasal cavity and paranasal sinuses characterized by unfavorable outcome, difficult treatment, diagnosis and prognosis. MicroRNAs are key molecules in the regulation of development and progression of cancer and their expression profiles could be used as prognostic biomarkers, to predict patients&apos; survival and response to treatment. In this study, we used quantitative real time PCR with TaqMan(R) Advanced miRNA Assays to investigate relative expression values of selected microRNAs in a unique set of formalin-fixed paraffin-embedded tissue samples obtained from 46 patients with sinonasal squamous cell carcinoma. Our results showed statistically significant upregulation of three mature microRNAs: miR-9-5p (fold change: 6.80), miR-9-3p (fold change: 3.07) and let-7d (fold change: 3.93) sinonasal carcinoma patients. Kaplan-Meier survival analysis and Logrank test identified connection between higher expression of miR-9-5p and longer survival of the patients (P = 0.0264). Lower expression of let-7d was detected in the patients with impaired survival and higher expression of miR-137 was linked to shorter survival of the patients. We also identified several correlations between expression of studied microRNAs and recorded clinicopathological data. Higher expression of miR-137, lower expression of let-7d correlated with local recurrence (P = 0.045 and P = 0.025), lower expression of miR-9-5p and higher expression of miR-155-5p correlated with regional recurrence (P = 0.045 and P = 0.036). Higher expression of miR-9-3p correlated with occupational risk (P = 0.031), presence of vascular invasion (P = 0.013) and perineural invasion (P = 0.031). Higher expression of miR-155-5p was present in the samples originating from maxillary sinus (P = 0.011), cN1-3 classified tumors (P = 0.009) and G2-3 classified tumors (P = 0.017). In conclusion, our study supports the hypothesis of future prospect to use miRNA expression as prognostic biomarkers of squamous cell sinonasal carcinoma. Especially, miR-9-5p and miR-9-3p seem to be important members of sinonasal cancer pathogenesis.

Název v anglickém jazyce

Deregulation of selected microRNAs in sinonasal squamous cell Carcinoma: Searching for Potential Prognostic Biomarkers

Popis výsledku anglicky

Sinonasal carcinomas are head and neck tumors arising from nasal cavity and paranasal sinuses characterized by unfavorable outcome, difficult treatment, diagnosis and prognosis. MicroRNAs are key molecules in the regulation of development and progression of cancer and their expression profiles could be used as prognostic biomarkers, to predict patients&apos; survival and response to treatment. In this study, we used quantitative real time PCR with TaqMan(R) Advanced miRNA Assays to investigate relative expression values of selected microRNAs in a unique set of formalin-fixed paraffin-embedded tissue samples obtained from 46 patients with sinonasal squamous cell carcinoma. Our results showed statistically significant upregulation of three mature microRNAs: miR-9-5p (fold change: 6.80), miR-9-3p (fold change: 3.07) and let-7d (fold change: 3.93) sinonasal carcinoma patients. Kaplan-Meier survival analysis and Logrank test identified connection between higher expression of miR-9-5p and longer survival of the patients (P = 0.0264). Lower expression of let-7d was detected in the patients with impaired survival and higher expression of miR-137 was linked to shorter survival of the patients. We also identified several correlations between expression of studied microRNAs and recorded clinicopathological data. Higher expression of miR-137, lower expression of let-7d correlated with local recurrence (P = 0.045 and P = 0.025), lower expression of miR-9-5p and higher expression of miR-155-5p correlated with regional recurrence (P = 0.045 and P = 0.036). Higher expression of miR-9-3p correlated with occupational risk (P = 0.031), presence of vascular invasion (P = 0.013) and perineural invasion (P = 0.031). Higher expression of miR-155-5p was present in the samples originating from maxillary sinus (P = 0.011), cN1-3 classified tumors (P = 0.009) and G2-3 classified tumors (P = 0.017). In conclusion, our study supports the hypothesis of future prospect to use miRNA expression as prognostic biomarkers of squamous cell sinonasal carcinoma. Especially, miR-9-5p and miR-9-3p seem to be important members of sinonasal cancer pathogenesis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

<a href="/cs/project/EF16_013%2F0001674" target="_blank" >EF16_013/0001674: BBMRI-CZ: Síť biobank - univerzální platforma k výzkumu etiopatogeneze chorob</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica

ISSN

0015-5500

e-ISSN

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Svazek periodika

65

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

142-151

Kód UT WoS článku

000500271800004

EID výsledku v databázi Scopus

2-s2.0-85073656014