Chondrosarcoma with Target-Like Chondrocytes: Update on Molecular Profiling and Specific Morphological Features

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F22%3A10451462" target="_blank" >RIV/00064165:_____/22:10451462 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064203:_____/22:10451462 RIV/00216208:11110/22:10451462

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=TqTRtz1gmT" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=TqTRtz1gmT</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chondrosarcoma with Target-Like Chondrocytes: Update on Molecular Profiling and Specific Morphological Features

Popis výsledku v původním jazyce

This is the first histological and molecular analysis of two chondrosarcomas with target-like chondrocytes that were compared with a group of conventional chondrosarcomas and enchondromas. The unique histological feature of target-like chon-drocytes is the presence of unusual hypertrophic eo-sinophilic APAS-positive perichondrocytic rings (baskets). In the sections stained with Safranin O/Fast green, the outer part of the ring was blue and the material in the lacunar space stained orange, simi-larly to intercellular regions. Immunohistoche mical examination showed strong positivity for vimentin, factor XIIIa, cyclin D1, osteonectin, B-cell lympho-ma 2 apoptosis regulator (Bcl-2), p53 and p16. The S-100 protein was positive in 25 % of neoplastic cells. Antibodies against GFAP, D2-40 (podoplanin), CD99, CKAE1.3 and CD10 exhibited weak focal positivity. Pericellular rings/baskets contained type VI collagen in their peripheral part, in contrast to the type II col-lagen in intercellular interterritorial spaces. Ultra -structural examination revealed that pericellular rings contained an intralacunar component com-posed of microfibrils with abundant admixture of aggregates of dense amorphous non-fibrillar mate-rial. The outer extralacunar zone was made up of a layer of condensed thin collagen fibrils with admix-ture of non-fibrillar dense material. NGS sequencing identified a fusion transcript involving fibronectin 1 (FN1) and fibroblast growth factor receptor 2 (FGFR2) at the RNA level. At the DNA level, no significant va-riant was revealed except for the presumably germ -line variant in the SPTA1 gene.

Název v anglickém jazyce

Chondrosarcoma with Target-Like Chondrocytes: Update on Molecular Profiling and Specific Morphological Features

Popis výsledku anglicky

This is the first histological and molecular analysis of two chondrosarcomas with target-like chondrocytes that were compared with a group of conventional chondrosarcomas and enchondromas. The unique histological feature of target-like chon-drocytes is the presence of unusual hypertrophic eo-sinophilic APAS-positive perichondrocytic rings (baskets). In the sections stained with Safranin O/Fast green, the outer part of the ring was blue and the material in the lacunar space stained orange, simi-larly to intercellular regions. Immunohistoche mical examination showed strong positivity for vimentin, factor XIIIa, cyclin D1, osteonectin, B-cell lympho-ma 2 apoptosis regulator (Bcl-2), p53 and p16. The S-100 protein was positive in 25 % of neoplastic cells. Antibodies against GFAP, D2-40 (podoplanin), CD99, CKAE1.3 and CD10 exhibited weak focal positivity. Pericellular rings/baskets contained type VI collagen in their peripheral part, in contrast to the type II col-lagen in intercellular interterritorial spaces. Ultra -structural examination revealed that pericellular rings contained an intralacunar component com-posed of microfibrils with abundant admixture of aggregates of dense amorphous non-fibrillar mate-rial. The outer extralacunar zone was made up of a layer of condensed thin collagen fibrils with admix-ture of non-fibrillar dense material. NGS sequencing identified a fusion transcript involving fibronectin 1 (FN1) and fibroblast growth factor receptor 2 (FGFR2) at the RNA level. At the DNA level, no significant va-riant was revealed except for the presumably germ -line variant in the SPTA1 gene.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30109 - Pathology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica

ISSN

0015-5500

e-ISSN

2533-7602

Svazek periodika

68

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

13

Strana od-do

112-124

Kód UT WoS článku

000908057400004

EID výsledku v databázi Scopus

2-s2.0-85147046451