Morphological and gene-expression characterization of viable heterogeneous circulating tumor cells size-captured and cultured from triple-negative breast cancer mouse models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F16%3AN0000117" target="_blank" >RIV/00064173:_____/16:N0000117 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/16:10327685 RIV/00064203:_____/16:10327685

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Morphological and gene-expression characterization of viable heterogeneous circulating tumor cells size-captured and cultured from triple-negative breast cancer mouse models

Popis výsledku v původním jazyce

The goal of the present study was to capture and characterize circulating tumor cells (CTC) from mouse models of triple-negative breast cancer (TNBC), a highly recalcitrant disease. CTCs were captured by size-based filtration from orthotopic nude-mouse models of the human MDA-MB-231-GFP cell line. Subsequently, CTCs were cultured for a 3 months period in vitro. Cyto-morphological analysis with confocal microscopy demonstrated heterogeneity among the CTCs, which alternated between adherent in culture. CTC gene-expression analysis was performed with qPCR for 20 tumor-associated markers from RNA derived from adherent/nonadherent CTCs in culture. Both Fox3p and TWIST1 were highly expressed in non-adherent CTCs. The present study demonstrates the power of size-based CTC isolation from orthotopic mouse models of TNBC and GFP imaging of viable CTC cells in culture, to characterize highly-heterogeneous TNBC CTC.

Název v anglickém jazyce

Morphological and gene-expression characterization of viable heterogeneous circulating tumor cells size-captured and cultured from triple-negative breast cancer mouse models

Popis výsledku anglicky

The goal of the present study was to capture and characterize circulating tumor cells (CTC) from mouse models of triple-negative breast cancer (TNBC), a highly recalcitrant disease. CTCs were captured by size-based filtration from orthotopic nude-mouse models of the human MDA-MB-231-GFP cell line. Subsequently, CTCs were cultured for a 3 months period in vitro. Cyto-morphological analysis with confocal microscopy demonstrated heterogeneity among the CTCs, which alternated between adherent in culture. CTC gene-expression analysis was performed with qPCR for 20 tumor-associated markers from RNA derived from adherent/nonadherent CTCs in culture. Both Fox3p and TWIST1 were highly expressed in non-adherent CTCs. The present study demonstrates the power of size-based CTC isolation from orthotopic mouse models of TNBC and GFP imaging of viable CTC cells in culture, to characterize highly-heterogeneous TNBC CTC.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT14441" target="_blank" >NT14441: Cirkulující a diseminované nádorové buňky v diagnostice a léčbě pokročilých stadií karcinomu ovaria</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Clinical and Experimental Medicine

ISSN

1940-5901

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

7772-7779

Kód UT WoS článku

000379956500015

EID výsledku v databázi Scopus

2-s2.0-84971542844