Circulating Endometrial Cells in Women With Spontaneous Pneumothorax

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F19%3AN0000030" target="_blank" >RIV/00064173:_____/19:N0000030 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61383082:_____/20:00000964 RIV/00216208:11110/20:10402507 RIV/00216208:11120/20:43918760 RIV/00064203:_____/20:10402507

Výsledek na webu

<a href="https://doi.org/10.1016/j.chest.2019.09.008" target="_blank" >https://doi.org/10.1016/j.chest.2019.09.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chest.2019.09.008" target="_blank" >10.1016/j.chest.2019.09.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Circulating Endometrial Cells in Women With Spontaneous Pneumothorax

Popis výsledku v původním jazyce

BACKGROUND: The occurrence of catamenial pneumothorax (CP) is rare and the awareness of this diagnosis among clinicians is insufficient. CP is highly correlated with pelvic endometriosis and remains the most common form of thoracic endometriosis syndrome. Circulating Endometrial Cells (CECs) were previously detected in patients with pelvic endometriosis. Could CECs bring new insights into pneumothorax management? METHODS: The presented study aims to describe the occurrence and molecular characteristics of CECs in women with spontaneous pneumothorax (SP) (n= 20) with high suspicion of its catamenial character. CECs were enriched from peripheral blood by size-based separation (MetaCell(R)). In addition to cytomorphology, gene expression profiling of captured cells was performed for 24 endometriosis associated genes. RESULTS: CECs were present in all 20 patients with SP. Enriched CECs exhibited four character features: epithelial, stem -cell like, stroma-like, and glandular. However, not all of them were present in every sampling. Gene expression profiling revealed two distinct phenotypes of CECs in SP/CP, whereby one of them refers to the diaphragm openings syndrome and the second to the endometrial tissue pleural implantations. Comparisons of the gene expression profiles of CECs in pneumothorax (CECs-SP) with CECs in pelvic endometriosis (CECs-non-SP) have revealed significantly higher expression of HER2 in CECs-SP compared to CECs-non-SP. CONCLUSION: This proof of concept study demonstrates successful isolation and characterization of CECs in patients with SP. Identification of CECs in SP could alert endometriosis involvement and help early referral to gynecologic consultation for further examination and treatment.

Název v anglickém jazyce

Circulating Endometrial Cells in Women With Spontaneous Pneumothorax

Popis výsledku anglicky

BACKGROUND: The occurrence of catamenial pneumothorax (CP) is rare and the awareness of this diagnosis among clinicians is insufficient. CP is highly correlated with pelvic endometriosis and remains the most common form of thoracic endometriosis syndrome. Circulating Endometrial Cells (CECs) were previously detected in patients with pelvic endometriosis. Could CECs bring new insights into pneumothorax management? METHODS: The presented study aims to describe the occurrence and molecular characteristics of CECs in women with spontaneous pneumothorax (SP) (n= 20) with high suspicion of its catamenial character. CECs were enriched from peripheral blood by size-based separation (MetaCell(R)). In addition to cytomorphology, gene expression profiling of captured cells was performed for 24 endometriosis associated genes. RESULTS: CECs were present in all 20 patients with SP. Enriched CECs exhibited four character features: epithelial, stem -cell like, stroma-like, and glandular. However, not all of them were present in every sampling. Gene expression profiling revealed two distinct phenotypes of CECs in SP/CP, whereby one of them refers to the diaphragm openings syndrome and the second to the endometrial tissue pleural implantations. Comparisons of the gene expression profiles of CECs in pneumothorax (CECs-SP) with CECs in pelvic endometriosis (CECs-non-SP) have revealed significantly higher expression of HER2 in CECs-SP compared to CECs-non-SP. CONCLUSION: This proof of concept study demonstrates successful isolation and characterization of CECs in patients with SP. Identification of CECs in SP could alert endometriosis involvement and help early referral to gynecologic consultation for further examination and treatment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chest

ISSN

0012-3692

e-ISSN

1931-3543

Svazek periodika

157

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

342-355

Kód UT WoS článku

000512414700023

EID výsledku v databázi Scopus

2-s2.0-85078156205