Assessment of Faecal Microbiota Transplant Stability in Deep-Freeze Conditions: A 12-Month Ex Vivo Viability Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F24%3A43926888" target="_blank" >RIV/00064173:_____/24:43926888 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/24:43926888

Výsledek na webu

<a href="https://doi.org/10.1002/jcla.25023" target="_blank" >https://doi.org/10.1002/jcla.25023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcla.25023" target="_blank" >10.1002/jcla.25023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of Faecal Microbiota Transplant Stability in Deep-Freeze Conditions: A 12-Month Ex Vivo Viability Analysis

Popis výsledku v původním jazyce

Background: Faecal microbiota transplantation (FMT) is an established treatment for Clostridioides difficile infection and is under investigation for other conditions. The availability of suitable donors and the logistics of fresh stool preparation present challenges, making frozen, biobanked stools an attractive alternative. Aims: This study aimed to evaluate the long-term viability of bacterial populations in faecal samples stored at -80oC for up to 12 months, supporting the feasibility of using frozen grafts for FMT. Methods: Fifteen faecal samples from nine healthy donors were processed, mixed with cryoprotectants and stored at -80oC. Samples were assessed at baseline and after 3, 6 and 12 months using quantitative culturing methods to determine the concentration of live bacteria. Results: Quantitative analysis showed no significant decrease in bacterial viability over the 12-month period for both aerobic and anaerobic cultures (p = 0.09). At all timepoints, the coefficients of variability in colony-forming unit (CFU) counts were greater between samples (102 +- 21% and 100 +- 13% for aerobic and anaerobic cultures, respectively) than the variability between measurements of the same sample (30 +- 22% and 30 +- 19%). Conclusions: The study confirmed that faecal microbiota can be preserved with high viability in deep-freeze storage for up to a year, making allogenic FMT from biobanked samples a viable and safer option for patients. However, a multidonor approach may be beneficial to mitigate the risk of viability loss in any single donor sample.

Název v anglickém jazyce

Assessment of Faecal Microbiota Transplant Stability in Deep-Freeze Conditions: A 12-Month Ex Vivo Viability Analysis

Popis výsledku anglicky

Background: Faecal microbiota transplantation (FMT) is an established treatment for Clostridioides difficile infection and is under investigation for other conditions. The availability of suitable donors and the logistics of fresh stool preparation present challenges, making frozen, biobanked stools an attractive alternative. Aims: This study aimed to evaluate the long-term viability of bacterial populations in faecal samples stored at -80oC for up to 12 months, supporting the feasibility of using frozen grafts for FMT. Methods: Fifteen faecal samples from nine healthy donors were processed, mixed with cryoprotectants and stored at -80oC. Samples were assessed at baseline and after 3, 6 and 12 months using quantitative culturing methods to determine the concentration of live bacteria. Results: Quantitative analysis showed no significant decrease in bacterial viability over the 12-month period for both aerobic and anaerobic cultures (p = 0.09). At all timepoints, the coefficients of variability in colony-forming unit (CFU) counts were greater between samples (102 +- 21% and 100 +- 13% for aerobic and anaerobic cultures, respectively) than the variability between measurements of the same sample (30 +- 22% and 30 +- 19%). Conclusions: The study confirmed that faecal microbiota can be preserved with high viability in deep-freeze storage for up to a year, making allogenic FMT from biobanked samples a viable and safer option for patients. However, a multidonor approach may be beneficial to mitigate the risk of viability loss in any single donor sample.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30221 - Critical care medicine and Emergency medicine

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Clinical Laboratory Analysis

ISSN

0887-8013

e-ISSN

1098-2825

Svazek periodika

38

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

"e25023"

Kód UT WoS článku

001191997800001

EID výsledku v databázi Scopus

2-s2.0-85189533145