Electrospun poly(L-lactide-co-DL-lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F24%3A43927086" target="_blank" >RIV/00064173:_____/24:43927086 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/24:00586028 RIV/00216208:11110/24:10480836 RIV/00216208:11120/24:43927086

Výsledek na webu

<a href="https://doi.org/10.1016/j.heliyon.2024.e30970" target="_blank" >https://doi.org/10.1016/j.heliyon.2024.e30970</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.heliyon.2024.e30970" target="_blank" >10.1016/j.heliyon.2024.e30970</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrospun poly(L-lactide-co-DL-lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

Popis výsledku v původním jazyce

Ultrathin electrospun poly (L-lactide-co-DL-lactide) nanofibrous membranes coated with fibronectin were explored as scaffolds for the ex vivo cultivation of limbal epithelial cells (LECs) for the treatment of limbal stem cell deficiency. The developed scaffolds were compared with the &quot;gold-standard&quot; fibrin gel. The resulting membranes composed of nanofibers possessed a very low thickness of 4 μm and allowed very good optical transparency in the wet state. The fibronectin-coated nanofibrous scaffolds demonstrated LEC expansion and successful cultivation similar to that on fibrin gel. Unlike the regular cobblestone epithelial cell morphology on the fibrin gel, the nanofibrous scaffold presented a mostly irregular epithelial morphology with a shift to a mesenchymal phenotype, as confirmed by the upregulation of profibroblastic genes: ACTA2 (p = 0.023), FBLN1 (p &lt; 0.001), and THY1 (p &lt; 0.001). Both culture conditions revealed comparable expression of stem cell markers, including KLF4, ΔNp63α and ABCG2, emphasizing the promise of polylactide-based nanofibrous membranes for further investigations.

Název v anglickém jazyce

Electrospun poly(L-lactide-co-DL-lactide) nanofibrous scaffold as substrate for ex vivo limbal epithelial cell cultivation

Popis výsledku anglicky

Ultrathin electrospun poly (L-lactide-co-DL-lactide) nanofibrous membranes coated with fibronectin were explored as scaffolds for the ex vivo cultivation of limbal epithelial cells (LECs) for the treatment of limbal stem cell deficiency. The developed scaffolds were compared with the &quot;gold-standard&quot; fibrin gel. The resulting membranes composed of nanofibers possessed a very low thickness of 4 μm and allowed very good optical transparency in the wet state. The fibronectin-coated nanofibrous scaffolds demonstrated LEC expansion and successful cultivation similar to that on fibrin gel. Unlike the regular cobblestone epithelial cell morphology on the fibrin gel, the nanofibrous scaffold presented a mostly irregular epithelial morphology with a shift to a mesenchymal phenotype, as confirmed by the upregulation of profibroblastic genes: ACTA2 (p = 0.023), FBLN1 (p &lt; 0.001), and THY1 (p &lt; 0.001). Both culture conditions revealed comparable expression of stem cell markers, including KLF4, ΔNp63α and ABCG2, emphasizing the promise of polylactide-based nanofibrous membranes for further investigations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30207 - Ophthalmology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Heliyon

ISSN

2405-8440

e-ISSN

2405-8440

Svazek periodika

10

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

"e30970"

Kód UT WoS článku

001299504800001

EID výsledku v databázi Scopus

2-s2.0-85193289511