Increased neuronal Rab5 immunoreactive endosomes do not colocalize with TDP- 43 in Motor Neuron Disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F10%3A%230000024" target="_blank" >RIV/00064190:_____/10:#0000024 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064190:_____/10:#0000145

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Increased neuronal Rab5 immunoreactive endosomes do not colocalize with TDP- 43 in Motor Neuron Disease

Popis výsledku v původním jazyce

In the present study, using a neuron-specific morphometric approach, we examined the expression of the early endosomal marker Rab5 and lysosomal cathepsins B, D, F, and L as well as PAS-stained structures. This was compared with the expression of ubiquitin, p62 and TDP-43 and its phosphorylated form. The principal finding was the increased expression of the endosomal marker Rab5 and lysosomal cathepsin D, and of PAS-positive structures in motor neurons of MND cases. Furthermore, the area-portion of Rab5immunoreactivity correlated well with the intracellular accumulation of ubiquitin, p62 and (phosphorylated) TDP-43. However, double immunolabelling and immunogold electron microscopy excluded co-localization of phosphorylated TDP-43 with the ELS. Thesedata contrast with observations on neuronal cytopathology in Alzheimer`s or prion diseases where the disease-specific proteins are processed withing endosomes, and suggest a distinct role of the ELS in MND.

Název v anglickém jazyce

Increased neuronal Rab5 immunoreactive endosomes do not colocalize with TDP- 43 in Motor Neuron Disease

Popis výsledku anglicky

In the present study, using a neuron-specific morphometric approach, we examined the expression of the early endosomal marker Rab5 and lysosomal cathepsins B, D, F, and L as well as PAS-stained structures. This was compared with the expression of ubiquitin, p62 and TDP-43 and its phosphorylated form. The principal finding was the increased expression of the endosomal marker Rab5 and lysosomal cathepsin D, and of PAS-positive structures in motor neurons of MND cases. Furthermore, the area-portion of Rab5immunoreactivity correlated well with the intracellular accumulation of ubiquitin, p62 and (phosphorylated) TDP-43. However, double immunolabelling and immunogold electron microscopy excluded co-localization of phosphorylated TDP-43 with the ELS. Thesedata contrast with observations on neuronal cytopathology in Alzheimer`s or prion diseases where the disease-specific proteins are processed withing endosomes, and suggest a distinct role of the ELS in MND.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/NR8491" target="_blank" >NR8491: Určení vztahu mezi formou amyotrofické laterální sklerózy a stupněm kognitivní alterace, markery neurodegenerace a mozkové atrofie - prospektivní studie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Neurology

ISSN

0014-4886

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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