Detection of immune cell response to M. tuberculosis-specific antigens by quantitative polymerase chain reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F12%3A%230000203" target="_blank" >RIV/00064190:_____/12:#0000203 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/12:00379056 RIV/61388971:_____/12:00379056 RIV/60461373:22340/12:43893185 RIV/00064190:_____/12:#0000314

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.diagmicrobio.2011.09.024" target="_blank" >http://dx.doi.org/10.1016/j.diagmicrobio.2011.09.024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diagmicrobio.2011.09.024" target="_blank" >10.1016/j.diagmicrobio.2011.09.024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of immune cell response to M. tuberculosis-specific antigens by quantitative polymerase chain reaction

Popis výsledku v původním jazyce

One third of the world's population is latently infected with Mycobacterium tuberculosis (Mtb) and up to 10% of infected individuals develop active tuberculosis (TB) in their lifetime. Among the major challenges in the control of TB is the implementationof sensitive methods for detection of latent tuberculosis infection (LTBI). Currently, in vitro interferon gamma release assays, yielding single value readout, are used as an alternative to the traditional tuberculin skin test for the diagnosis of LTBI.More complex characterization of immune status of LTBI individuals, however, is desirable for indication of LTBI subjects for preventative chemotherapy. Here we describe a quantitative polymerase chain reaction (qPCR) for determination of expression levels of 14 genes, additional to interferon gamma, which was applied for comparison of the specific Mtb-antigen immune response of blood cells from healthy, latently infected, and TB individuals. With the use of principal component analysis

Název v anglickém jazyce

Detection of immune cell response to M. tuberculosis-specific antigens by quantitative polymerase chain reaction

Popis výsledku anglicky

One third of the world's population is latently infected with Mycobacterium tuberculosis (Mtb) and up to 10% of infected individuals develop active tuberculosis (TB) in their lifetime. Among the major challenges in the control of TB is the implementationof sensitive methods for detection of latent tuberculosis infection (LTBI). Currently, in vitro interferon gamma release assays, yielding single value readout, are used as an alternative to the traditional tuberculin skin test for the diagnosis of LTBI.More complex characterization of immune status of LTBI individuals, however, is desirable for indication of LTBI subjects for preventative chemotherapy. Here we describe a quantitative polymerase chain reaction (qPCR) for determination of expression levels of 14 genes, additional to interferon gamma, which was applied for comparison of the specific Mtb-antigen immune response of blood cells from healthy, latently infected, and TB individuals. With the use of principal component analysis

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FC - Pneumologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

ISSN

0732-8893

e-ISSN

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Svazek periodika

72

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

68-78

Kód UT WoS článku

000298444000010

EID výsledku v databázi Scopus

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