Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F24%3A10001226" target="_blank" >RIV/00064190:_____/24:10001226 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/24:10480437 RIV/00216208:11130/24:10480437

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1472979224000441?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1472979224000441?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.tube.2024.102518" target="_blank" >10.1016/j.tube.2024.102518</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease

Popis výsledku v původním jazyce

Authors present a pilot study of the development of innovative flow cytometry-based assay with a potential for use in tuberculosis diagnostics. Currently available tests do not provide robust discrimination between latent tuberculosis infection (TBI) and tuberculosis disease (TB). The desired application is to distinguish between the two conditions by evaluating the production of a combination of three cytokines: IL-2 (interleukin-2), IFN gamma (interferon gamma) and TNF alpha (tumor necrosis factor alpha) in CD4+ and CD8+ T cells. The study was conducted on 68 participants, divided into two arms according to age (paediatric and adults). Each arm was further split into three categories (non-infection (NI), TBI, TB) based on the immune reaction to Mycobacterium tuberculosis (M.tb) after a close contact with pulmonary TB. Each blood sample was stimulated with specific M.tb antigens present in QuantiFERON tubes (TB1 and TB2). We inferred TBI or TB based on the predominant cytokine response of the CD4+ and/or CD8+ T cells. Significant differences were detected between the NI, TBI and the TB groups in TB1 in the CD4+TNF alpha+parameter in children. Along with IL-2, TNF alpha seems to be the most promising diagnostic marker in both CD4+and CD8+ T cells. However, more detailed analyses on larger cohorts are needed to confirm the observed tendencies.

Název v anglickém jazyce

Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease

Popis výsledku anglicky

Authors present a pilot study of the development of innovative flow cytometry-based assay with a potential for use in tuberculosis diagnostics. Currently available tests do not provide robust discrimination between latent tuberculosis infection (TBI) and tuberculosis disease (TB). The desired application is to distinguish between the two conditions by evaluating the production of a combination of three cytokines: IL-2 (interleukin-2), IFN gamma (interferon gamma) and TNF alpha (tumor necrosis factor alpha) in CD4+ and CD8+ T cells. The study was conducted on 68 participants, divided into two arms according to age (paediatric and adults). Each arm was further split into three categories (non-infection (NI), TBI, TB) based on the immune reaction to Mycobacterium tuberculosis (M.tb) after a close contact with pulmonary TB. Each blood sample was stimulated with specific M.tb antigens present in QuantiFERON tubes (TB1 and TB2). We inferred TBI or TB based on the predominant cytokine response of the CD4+ and/or CD8+ T cells. Significant differences were detected between the NI, TBI and the TB groups in TB1 in the CD4+TNF alpha+parameter in children. Along with IL-2, TNF alpha seems to be the most promising diagnostic marker in both CD4+and CD8+ T cells. However, more detailed analyses on larger cohorts are needed to confirm the observed tendencies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30203 - Respiratory systems

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TUBERCULOSIS

ISSN

1472-9792

e-ISSN

1873-281X

Svazek periodika

147

Číslo periodika v rámci svazku

07/2024

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

001241064500001

EID výsledku v databázi Scopus

2-s2.0-85192966028