Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous I3-catenin and cytoplasmic I3-catenin expression with the outcome of patients with clear cell renal cell carcinoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F25%3A10001103" target="_blank" >RIV/00064190:_____/25:10001103 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/26:00564637 RIV/00216224:14110/25:00140234

Výsledek na webu

<a href="https://biomed.papers.upol.cz/corproof.php?tartkey=bio-000000-3573" target="_blank" >https://biomed.papers.upol.cz/corproof.php?tartkey=bio-000000-3573</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2024.005" target="_blank" >10.5507/bp.2024.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous I3-catenin and cytoplasmic I3-catenin expression with the outcome of patients with clear cell renal cell carcinoma

Popis výsledku v původním jazyce

Aims. The objective of this study was to investigate the association and combined prognostic significance of the PD -L1, Smoothened protein and beta-catenin expressions in patients with clear cell renal cell carcinoma (ccRCC). Methods.The PD -L1, Smoothened protein and beta-catenin expression were evaluated in 104 ccRCC patients. All studied tumor samples were acquired from nephrectomy specimens of primary tumors and not from biopsies or metastases. An indirect immunohistochemistry using polyclonal rabbit anti -Smoothened antibody, monoclonal mouse anti -human beta-catenin-1 antibody, immunohistochemical assay PD -L1 28-8 pharmDx using monoclonal rabbit anti -PD -L1 antibody and anti-VHL (C- terminal) rabbit antibody was used. Immunohistochemistry was scored semiquantitavely. Results. Median overall survival (OS) was significantly better in patients with lower PD -L1 expression (&lt;= 5%), Smoothened protein (SMO) expression (&lt;5%) or cytoplasmic beta-catenin expression (&lt;= 75%) than in patients with higher expressions of these biomarkers (P&lt;0.001, P=0.047, and P&lt;0.001, respectively). Membranous beta-catenin showed an opposite effect with its lower expression (&lt;= 75%) being associated with longer OS (P=0.020). There was significant association between PD -1 and PD -L1 expression (P=0.007) and significant association of tumor grade (WHO 2016) with membranous beta-catenin (P&lt;0.001), cytoplasmic beta-catenin (P=0.005), pVHL (P=0.042), PD -L1 (P=0.049) and PD -1 (P=0.028) expression. Conclusion. The present study provides the first data on the potential association and combined prognostic significance of frequency of primary cilia, PD -L1, Smoothened protein and beta-catenin expression with the outcome in clear cell renal cell carcinoma.

Název v anglickém jazyce

Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous I3-catenin and cytoplasmic I3-catenin expression with the outcome of patients with clear cell renal cell carcinoma

Popis výsledku anglicky

Aims. The objective of this study was to investigate the association and combined prognostic significance of the PD -L1, Smoothened protein and beta-catenin expressions in patients with clear cell renal cell carcinoma (ccRCC). Methods.The PD -L1, Smoothened protein and beta-catenin expression were evaluated in 104 ccRCC patients. All studied tumor samples were acquired from nephrectomy specimens of primary tumors and not from biopsies or metastases. An indirect immunohistochemistry using polyclonal rabbit anti -Smoothened antibody, monoclonal mouse anti -human beta-catenin-1 antibody, immunohistochemical assay PD -L1 28-8 pharmDx using monoclonal rabbit anti -PD -L1 antibody and anti-VHL (C- terminal) rabbit antibody was used. Immunohistochemistry was scored semiquantitavely. Results. Median overall survival (OS) was significantly better in patients with lower PD -L1 expression (&lt;= 5%), Smoothened protein (SMO) expression (&lt;5%) or cytoplasmic beta-catenin expression (&lt;= 75%) than in patients with higher expressions of these biomarkers (P&lt;0.001, P=0.047, and P&lt;0.001, respectively). Membranous beta-catenin showed an opposite effect with its lower expression (&lt;= 75%) being associated with longer OS (P=0.020). There was significant association between PD -1 and PD -L1 expression (P=0.007) and significant association of tumor grade (WHO 2016) with membranous beta-catenin (P&lt;0.001), cytoplasmic beta-catenin (P=0.005), pVHL (P=0.042), PD -L1 (P=0.049) and PD -1 (P=0.028) expression. Conclusion. The present study provides the first data on the potential association and combined prognostic significance of frequency of primary cilia, PD -L1, Smoothened protein and beta-catenin expression with the outcome in clear cell renal cell carcinoma.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/LX22NPO5102" target="_blank" >LX22NPO5102: Národní ústav pro výzkum rakoviny</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1213-8118

e-ISSN

1804-7521

Svazek periodika

169

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

107-115

Kód UT WoS článku

001179455000001

EID výsledku v databázi Scopus

2-s2.0-105008374155