An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6388" target="_blank" >RIV/00064203:_____/10:6388 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/10:00166514 RIV/62157124:16370/10:00002648 RIV/00216208:11130/10:6388

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics

Popis výsledku v původním jazyce

The drugs based on platinum metals represent effective groups of chemotherapeutic agents. It is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. Platinum based drugs resistance can also be connected with increased expression of metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction, and a comparison of results in vitro and in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed neuroblastoma lines treated with cisplatin or carboplatin. It is clear that cisplatin-resistant and cisplatin-sensitive lines unlikely respond to the presence of cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits and serum samples from patients with retinoblastoma treated with carboplatin.

Název v anglickém jazyce

An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics

Popis výsledku anglicky

The drugs based on platinum metals represent effective groups of chemotherapeutic agents. It is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. Platinum based drugs resistance can also be connected with increased expression of metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction, and a comparison of results in vitro and in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed neuroblastoma lines treated with cisplatin or carboplatin. It is clear that cisplatin-resistant and cisplatin-sensitive lines unlikely respond to the presence of cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits and serum samples from patients with retinoblastoma treated with carboplatin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Science

ISSN

1422-0067

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

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Kód UT WoS článku

000285708000004

EID výsledku v databázi Scopus

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