Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F13%3A10209799" target="_blank" >RIV/00064203:_____/13:10209799 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/13:43901647 RIV/00064173:_____/13:#0000295 RIV/00216208:11130/13:10209799

Výsledek na webu

<a href="http://dx.doi.org/10.5507/bp.2012.088" target="_blank" >http://dx.doi.org/10.5507/bp.2012.088</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2012.088" target="_blank" >10.5507/bp.2012.088</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing

Popis výsledku v původním jazyce

Aim. To compare aspects of wound healing after cleft lip surgery performed within one week of age and wound healing after surgery performed within 2 - 4 months of age, especially concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in tissue removed during surgery. Methods. 34 tissue samples (26 boys and 8 girls) were removed during surgery within one week of age (n=19) or within 2 - 4 months of age (n=15). Tissue samples were separated into epidermis, dermis and mucous membrane. Proteins were extracted in cacodylic buffer for 24 h at a temperature 2 - 8 degrees C. Total protein concentrations were examined using a modification of the Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-9 and TIMP-1 concentrations. Results. MMP-9: early surgery - epidermis 2.168+/-3.303 mu g/g of protein (mean+/-SD), dermis 1.251+/-1.848 mu g/g, 2 - 4 months surgery - epidermis 0

Název v anglickém jazyce

Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing

Popis výsledku anglicky

Aim. To compare aspects of wound healing after cleft lip surgery performed within one week of age and wound healing after surgery performed within 2 - 4 months of age, especially concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in tissue removed during surgery. Methods. 34 tissue samples (26 boys and 8 girls) were removed during surgery within one week of age (n=19) or within 2 - 4 months of age (n=15). Tissue samples were separated into epidermis, dermis and mucous membrane. Proteins were extracted in cacodylic buffer for 24 h at a temperature 2 - 8 degrees C. Total protein concentrations were examined using a modification of the Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-9 and TIMP-1 concentrations. Results. MMP-9: early surgery - epidermis 2.168+/-3.303 mu g/g of protein (mean+/-SD), dermis 1.251+/-1.848 mu g/g, 2 - 4 months surgery - epidermis 0

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/NS10012" target="_blank" >NS10012: Vliv časné operace rosštěpu rtu u novorozenců na hojení operační rány.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Svazek periodika

157

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

363-366

Kód UT WoS článku

000329091500015

EID výsledku v databázi Scopus

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