Structural changes in the bronchial mucosa of young children at risk of developing asthma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F14%3A10293025" target="_blank" >RIV/00064203:_____/14:10293025 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/14:10293025

Výsledek na webu

<a href="http://dx.doi.org/10.1111/pai.12119" target="_blank" >http://dx.doi.org/10.1111/pai.12119</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pai.12119" target="_blank" >10.1111/pai.12119</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural changes in the bronchial mucosa of young children at risk of developing asthma

Popis výsledku v původním jazyce

Background Allergen-specific immunotherapy (IT) is widely used to treat allergic diseases. The molecular mechanisms have not been clarified yet completely. The present work was undertaken to analyze the effect of IT in the activation of NF-kappa B. Methods Neutrophils from 15 pollen-allergic IT-treated patients, 10 untreated pollen-allergic patients, and 10 healthy donors were in vitro stimulated with LPS. NF-kappa B activation (p65/p52) was measured in their nuclear extracts by enzyme-linked immunosorbent assay (ELISA). I kappa B alpha phosphorylation, NF-kappa B-repressing factor (NRF) activation, and thromboxane A(2) (TXA(2)) and Interleukin-8 (IL-8) release were measured by ELISA. Results There was a positive correlation between the score of symptoms and NF-kappa B activation in human neutrophils. IT significantly decreased NF-kappa B activation levels in neutrophils compared with neutrophils from untreated patients. I kappa B alpha phosphorylation and NRF activation levels were, r

Název v anglickém jazyce

Structural changes in the bronchial mucosa of young children at risk of developing asthma

Popis výsledku anglicky

Background Allergen-specific immunotherapy (IT) is widely used to treat allergic diseases. The molecular mechanisms have not been clarified yet completely. The present work was undertaken to analyze the effect of IT in the activation of NF-kappa B. Methods Neutrophils from 15 pollen-allergic IT-treated patients, 10 untreated pollen-allergic patients, and 10 healthy donors were in vitro stimulated with LPS. NF-kappa B activation (p65/p52) was measured in their nuclear extracts by enzyme-linked immunosorbent assay (ELISA). I kappa B alpha phosphorylation, NF-kappa B-repressing factor (NRF) activation, and thromboxane A(2) (TXA(2)) and Interleukin-8 (IL-8) release were measured by ELISA. Results There was a positive correlation between the score of symptoms and NF-kappa B activation in human neutrophils. IT significantly decreased NF-kappa B activation levels in neutrophils compared with neutrophils from untreated patients. I kappa B alpha phosphorylation and NRF activation levels were, r

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FG - Pediatrie

OECD FORD obor

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Projekt

<a href="/cs/project/NT11444" target="_blank" >NT11444: Časné projevy průduškového astmatu u dětí - funkční a morfologické důsledky nemoci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pediatric Allergy and Immunology

ISSN

0905-6157

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

7

Strana od-do

136-142

Kód UT WoS článku

000332968000005

EID výsledku v databázi Scopus

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