How and why to monitor Pseudomonas aeruginosa infections in the long term at a cystic fibrosis centre

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F16%3A10323762" target="_blank" >RIV/00064203:_____/16:10323762 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/16:10323762

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jhin.2015.09.010" target="_blank" >http://dx.doi.org/10.1016/j.jhin.2015.09.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jhin.2015.09.010" target="_blank" >10.1016/j.jhin.2015.09.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

How and why to monitor Pseudomonas aeruginosa infections in the long term at a cystic fibrosis centre

Popis výsledku v původním jazyce

Background: Pseudomonas aeruginosa is a major cystic fibrosis (CF) pathogen causing chronic respiratory infections and posing a risk for cross-infection between patients with CF. Aim: To propose an algorithm for long-term surveillance of P. aeruginosa and assess its suitability for monitoring the epidemiological situation at a CF centre with approximately 300 patients. Methods: Over a nine-year period, over 300 P. aeruginosa isolates from 131 infected patients were tested by multi-locus sequence typing (MLST) and/or random amplified polymorphic DNA (RAPD) assay. Findings: MLST analysis led to the identification of 97 different sequence types which were distributed among 17 RAPD-generated (pseudo) clusters. This indicates that the easy-to-perform RAPD assay is only suitable for intra-individual, not interindividual, strain analyses. No epidemic strains were observed. Longitudinal analysis revealed that 110 of the 131 patients were infected with the same strain over the observation period, whereas 21 patients had a strain replacement or a new infection. Chronic infection was found in 99 of the 131 patients, and the remaining 32 patients met the criteria for intermittent infection (as defined by the Leeds criteria). Eighteen of the 32 patients (56%) with intermittent infection were infected with the same strain for up to nine years. Conclusion: The strain type only changed in 16% of 131 patients with chronic or intermittent infection. As many as 56% of patients considered to have intermittent infection were actually chronically infected with the same strain for many years.

Název v anglickém jazyce

How and why to monitor Pseudomonas aeruginosa infections in the long term at a cystic fibrosis centre

Popis výsledku anglicky

Background: Pseudomonas aeruginosa is a major cystic fibrosis (CF) pathogen causing chronic respiratory infections and posing a risk for cross-infection between patients with CF. Aim: To propose an algorithm for long-term surveillance of P. aeruginosa and assess its suitability for monitoring the epidemiological situation at a CF centre with approximately 300 patients. Methods: Over a nine-year period, over 300 P. aeruginosa isolates from 131 infected patients were tested by multi-locus sequence typing (MLST) and/or random amplified polymorphic DNA (RAPD) assay. Findings: MLST analysis led to the identification of 97 different sequence types which were distributed among 17 RAPD-generated (pseudo) clusters. This indicates that the easy-to-perform RAPD assay is only suitable for intra-individual, not interindividual, strain analyses. No epidemic strains were observed. Longitudinal analysis revealed that 110 of the 131 patients were infected with the same strain over the observation period, whereas 21 patients had a strain replacement or a new infection. Chronic infection was found in 99 of the 131 patients, and the remaining 32 patients met the criteria for intermittent infection (as defined by the Leeds criteria). Eighteen of the 32 patients (56%) with intermittent infection were infected with the same strain for up to nine years. Conclusion: The strain type only changed in 16% of 131 patients with chronic or intermittent infection. As many as 56% of patients considered to have intermittent infection were actually chronically infected with the same strain for many years.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/NT12405" target="_blank" >NT12405: Studium patogeneze smrtelné infekční komplikace způsobené bakterií Burkholderia cenocepacia u pacientů s cystickou fibrózou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Hospital Infection

ISSN

0195-6701

e-ISSN

—

Svazek periodika

92

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

54-60

Kód UT WoS článku

000367619800012

EID výsledku v databázi Scopus

2-s2.0-84952981381