Molecular characterisation of Czech Clostridium difficile isolates collected in 2013-2015

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F16%3A10332578" target="_blank" >RIV/00064203:_____/16:10332578 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/16:10332578

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijmm.2016.07.003" target="_blank" >http://dx.doi.org/10.1016/j.ijmm.2016.07.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijmm.2016.07.003" target="_blank" >10.1016/j.ijmm.2016.07.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterisation of Czech Clostridium difficile isolates collected in 2013-2015

Popis výsledku v původním jazyce

Clostridium difficile is a leading nosocomial pathogen and molecular typing is a crucial part of monitoring its occurrence and spread. Over a three-year period (2013-2015), clinical C difficile isolates from 32 Czech hospitals were collected for molecular characterisation. Of 2201 C difficile isolates, 177 (8%) were non-toxigenic, 2024 (92%) were toxigenic (tcdA and tcdB) and of these, 677 (33.5%) carried genes for binary toxin production (cdtA, cdtB). Capillary-electrophoresis (CE) ribotyping of the 2201 isolates yielded 166 different CE-ribotyping profiles, of which 53 were represented by at least two isolates for each profile. Of these, 29 CE-ribotyping patterns were common to the Leeds-Leiden C. difficile reference strain library and the WEBRIBO database (83.7% isolates), and 24 patterns were recognized only by the WEBRIBO database (11.2% isolates). Isolates belonging to these 53 CE-ribotyping profiles comprised 94.9% of all isolates. The ten most frequent CE-ribotyping profiles were 176 (n=588, 26.7%), 001 (n = 456, 20.7%), 014 (n=176, 8%), 012 (n =127, 5.8%), 017 (n =85,3.9%), 020 (n= 68, 3.1%), 596 (n = 55, 2.5%), 002-like (n = 45, 2.1%), 010 (n =35, 1.6%) and 078 (n=34, 1.6%). Multi-locus sequence typing (MIST) of seven housekeeping genes performed in one isolate of each of 53 different CE-ribotyping profiles revealed 40 different sequence types (STs). We conclude that molecular characterisation of Czech C difficile isolates revealed a high diversity of CE-ribotyping profiles; the prevailing RTs were 001 (20.7%) and 176 (027-like, 26.7%).

Název v anglickém jazyce

Molecular characterisation of Czech Clostridium difficile isolates collected in 2013-2015

Popis výsledku anglicky

Clostridium difficile is a leading nosocomial pathogen and molecular typing is a crucial part of monitoring its occurrence and spread. Over a three-year period (2013-2015), clinical C difficile isolates from 32 Czech hospitals were collected for molecular characterisation. Of 2201 C difficile isolates, 177 (8%) were non-toxigenic, 2024 (92%) were toxigenic (tcdA and tcdB) and of these, 677 (33.5%) carried genes for binary toxin production (cdtA, cdtB). Capillary-electrophoresis (CE) ribotyping of the 2201 isolates yielded 166 different CE-ribotyping profiles, of which 53 were represented by at least two isolates for each profile. Of these, 29 CE-ribotyping patterns were common to the Leeds-Leiden C. difficile reference strain library and the WEBRIBO database (83.7% isolates), and 24 patterns were recognized only by the WEBRIBO database (11.2% isolates). Isolates belonging to these 53 CE-ribotyping profiles comprised 94.9% of all isolates. The ten most frequent CE-ribotyping profiles were 176 (n=588, 26.7%), 001 (n = 456, 20.7%), 014 (n=176, 8%), 012 (n =127, 5.8%), 017 (n =85,3.9%), 020 (n= 68, 3.1%), 596 (n = 55, 2.5%), 002-like (n = 45, 2.1%), 010 (n =35, 1.6%) and 078 (n=34, 1.6%). Multi-locus sequence typing (MIST) of seven housekeeping genes performed in one isolate of each of 53 different CE-ribotyping profiles revealed 40 different sequence types (STs). We conclude that molecular characterisation of Czech C difficile isolates revealed a high diversity of CE-ribotyping profiles; the prevailing RTs were 001 (20.7%) and 176 (027-like, 26.7%).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Medical Microbiology

ISSN

1438-4221

e-ISSN

—

Svazek periodika

306

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

479-485

Kód UT WoS článku

000387522200001

EID výsledku v databázi Scopus

2-s2.0-84994268366