Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F24%3A10483764" target="_blank" >RIV/00064203:_____/24:10483764 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/24:10483764

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=kZ8ydrOR15" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=kZ8ydrOR15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/bs.mcb.2024.07.002" target="_blank" >10.1016/bs.mcb.2024.07.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors

Popis výsledku v původním jazyce

Plasmacytoid dendritic cells (pDCs) are a minority subset of dendritic cells that despite their tiny quantity play an important role in the immune system, especially in antiviral immunity. They are known mostly as the major producers of type I IFN, which they secrete upon stimulation of endosomal Toll-like receptors 7 and 9 with viral RNA and DNA. However, the functionality of pDCs is more complex, as they were shown to be also involved in autoimmunity, inflammation, and cancer. In the context of the tumor microenvironment, pDCs mostly show substantial functional defects and thus contribute to establishing immunosuppressive micromilieu. Indeed, tumor-infiltrating pDCs were shown to be predominantly pro-tumorigenic, with reduced ability to produce IFNα and capacity to prime regulatory T cells via the ICOS/ICOS-L pathway. Here we describe in detail a method to assess the functional capacity of pDCs upon exposure to tumor-derived cell culture supernatants. The same technique can be implemented with minimal variations to test any soluble factor&apos;s impact on pDC phenotype and function.

Název v anglickém jazyce

Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors

Popis výsledku anglicky

Plasmacytoid dendritic cells (pDCs) are a minority subset of dendritic cells that despite their tiny quantity play an important role in the immune system, especially in antiviral immunity. They are known mostly as the major producers of type I IFN, which they secrete upon stimulation of endosomal Toll-like receptors 7 and 9 with viral RNA and DNA. However, the functionality of pDCs is more complex, as they were shown to be also involved in autoimmunity, inflammation, and cancer. In the context of the tumor microenvironment, pDCs mostly show substantial functional defects and thus contribute to establishing immunosuppressive micromilieu. Indeed, tumor-infiltrating pDCs were shown to be predominantly pro-tumorigenic, with reduced ability to produce IFNα and capacity to prime regulatory T cells via the ICOS/ICOS-L pathway. Here we describe in detail a method to assess the functional capacity of pDCs upon exposure to tumor-derived cell culture supernatants. The same technique can be implemented with minimal variations to test any soluble factor&apos;s impact on pDC phenotype and function.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods in Cell Biology

ISSN

0091-679X

e-ISSN

—

Svazek periodika

2024

Číslo periodika v rámci svazku

189

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

85-96

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85201890179