Immunohistochemical expression of GPNMB in TFEB-rearranged and TFEB-amplified renal cell carcinomas

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064211%3A_____%2F26%3AW0000086" target="_blank" >RIV/00064211:_____/26:W0000086 - isvavai.cz</a>

Výsledek na webu

<a href="https://oadoi.org/10.1007/s00428-026-04466-6" target="_blank" >https://oadoi.org/10.1007/s00428-026-04466-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00428-026-04466-6" target="_blank" >10.1007/s00428-026-04466-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunohistochemical expression of GPNMB in TFEB-rearranged and TFEB-amplified renal cell carcinomas

Popis výsledku v původním jazyce

TFEB-altered renal cell carcinomas (RCCs) display a wide range of morphological features and variable immunohistochemical profiles, often overlapping with other RCC subtypes. This variability poses significant diagnostic challenges. Consequently, there is an ongoing search for reliable ancillary tests that can aid in identifying cases that warrant molecular testing. In this study, we focused on GPNMB immunohistochemistry expression in TFEB-rearranged and TFEB-amplified RCCs. A total of 25 TFEB-altered RCCs, including 16 TFEB-amplified RCCs and nine TFEB-rearranged RCCs, were included in the study. GPNMB immunohistochemistry was positive in all nine TFEB-rearranged RCCs, demonstrating strong, diffuse cytoplasmic staining. In the TFEB-amplified RCC cohort, GPNMB expression was also detected in all 16 tumors; however, the staining pattern differed from that seen in TFEB-rearranged RCCs. Diffuse staining was observed in eight tumors (50%), four of which showed variable staining intensity, while the remaining eight tumors (50%) exhibited focal GPNMB reactivity, with intervening negative areas. GPNMB was therefore positive in all 25/25 (100%) TFEB-altered RCCs by IHC, supporting its potential role as a sensitive screening marker for such RCCs. TFEB-rearranged RCCs typically exhibited strong, diffuse GPNMB immunoreactivity, whereas TFEB-amplified RCCs more often showed patchy or focal staining of lower intensity, interspersed with areas of GPNMB negativity. Variability of staining in TFEB-amplified RCCs underscores the need for cautious interpretation, particularly in limited biopsy specimens, taking into account the morphologic findings and other immunohistochemistry stains, such as Melan A and Cathepsin K.

Název v anglickém jazyce

Immunohistochemical expression of GPNMB in TFEB-rearranged and TFEB-amplified renal cell carcinomas

Popis výsledku anglicky

TFEB-altered renal cell carcinomas (RCCs) display a wide range of morphological features and variable immunohistochemical profiles, often overlapping with other RCC subtypes. This variability poses significant diagnostic challenges. Consequently, there is an ongoing search for reliable ancillary tests that can aid in identifying cases that warrant molecular testing. In this study, we focused on GPNMB immunohistochemistry expression in TFEB-rearranged and TFEB-amplified RCCs. A total of 25 TFEB-altered RCCs, including 16 TFEB-amplified RCCs and nine TFEB-rearranged RCCs, were included in the study. GPNMB immunohistochemistry was positive in all nine TFEB-rearranged RCCs, demonstrating strong, diffuse cytoplasmic staining. In the TFEB-amplified RCC cohort, GPNMB expression was also detected in all 16 tumors; however, the staining pattern differed from that seen in TFEB-rearranged RCCs. Diffuse staining was observed in eight tumors (50%), four of which showed variable staining intensity, while the remaining eight tumors (50%) exhibited focal GPNMB reactivity, with intervening negative areas. GPNMB was therefore positive in all 25/25 (100%) TFEB-altered RCCs by IHC, supporting its potential role as a sensitive screening marker for such RCCs. TFEB-rearranged RCCs typically exhibited strong, diffuse GPNMB immunoreactivity, whereas TFEB-amplified RCCs more often showed patchy or focal staining of lower intensity, interspersed with areas of GPNMB negativity. Variability of staining in TFEB-amplified RCCs underscores the need for cautious interpretation, particularly in limited biopsy specimens, taking into account the morphologic findings and other immunohistochemistry stains, such as Melan A and Cathepsin K.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30109 - Pathology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

VIRCHOWS ARCHIV

ISSN

0945-6317

e-ISSN

1432-2307

Svazek periodika

neuveden

Číslo periodika v rámci svazku

18 February 2026

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

nestrankovano

Kód UT WoS článku

001705999300001

EID výsledku v databázi Scopus

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