Determination of adenosine deaminase activity in human erythocytes by on-column Capillary isotachophoresis - capillary zone electrophoresis in the present of electrosmotic flow

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F99%3A00000003" target="_blank" >RIV/00098892:_____/99:00000003 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of adenosine deaminase activity in human erythocytes by on-column Capillary isotachophoresis - capillary zone electrophoresis in the present of electrosmotic flow

Popis výsledku v původním jazyce

Transient capillary isotachophoresis - capillary electrophoresis at the presenseof electroosmotic flow was utilized for the measurement of adenosine deaminase activity in human erythocytes. Phosphates, a dominant anios of the sample matrice were used asleading ions for transint isotachophoresis and borates (0.3 M, pH 10) were used as terminating ions and background electrolyte for capillary zone electrophoresis. Final experimental conditions allowed to inject 70% of the total capillary volume (1.45 mL)with the sample. Product of enzymatic conversions present in the sample in low-micromoral range were determined by the use of optimized conditions. Limit of detection was 28 nM using UV detection at 202 nm. Presented data showed that CITP-CZE can be performed in uncoated capillaries at the presence of strong EOF.

Název v anglickém jazyce

Determination of adenosine deaminase activity in human erythocytes by on-column Capillary isotachophoresis - capillary zone electrophoresis in the present of electrosmotic flow

Popis výsledku anglicky

Transient capillary isotachophoresis - capillary electrophoresis at the presenseof electroosmotic flow was utilized for the measurement of adenosine deaminase activity in human erythocytes. Phosphates, a dominant anios of the sample matrice were used asleading ions for transint isotachophoresis and borates (0.3 M, pH 10) were used as terminating ions and background electrolyte for capillary zone electrophoresis. Final experimental conditions allowed to inject 70% of the total capillary volume (1.45 mL)with the sample. Product of enzymatic conversions present in the sample in low-micromoral range were determined by the use of optimized conditions. Limit of detection was 28 nM using UV detection at 202 nm. Presented data showed that CITP-CZE can be performed in uncoated capillaries at the presence of strong EOF.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FG - Pediatrie

OECD FORD obor

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Projekt

<a href="/cs/project/IZ3439" target="_blank" >IZ3439: Diagnostika dědičných poruch purinového a pyrimidinového metabolismu pomocí kapilární elektroforézy.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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