CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061422" target="_blank" >RIV/00159816:_____/14:00061422 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081715:_____/14:00432067 RIV/00216224:14110/14:00073246

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00216-014-8053-8" target="_blank" >http://dx.doi.org/10.1007/s00216-014-8053-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-014-8053-8" target="_blank" >10.1007/s00216-014-8053-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices

Popis výsledku v původním jazyce

The effect of antibiotics on the microbial cells and concentration of antibiotics in the human body is essential for the effective use of antimicrobial therapy. The capillary isoelectric focusing is a suitable technique for the separation and the detection of bacteria, and amphoteric substances from nature. However, the determination of isoelectric points of ampholytic antibiotics by conventional techniques is time consuming. For this reason, capillary isoelectric focusing seems to be appropriate as a simple and reliable way for establishing them. The separation conditions for the capillary isoelectric focusing of selected ampholytic antibiotics with known isoelectric points and pK (a)s, ampicillin (pI 4.9), ciprofloxacin (pI 7.4), ofloxacin (pI 7.1),tetracycline (pI 5.4), tigecycline (pI 9.7), and vancomycin (pI 8.1), were found and optimized in the suitable pH ranges pH 2.0-5.3, 2.0-9.6, and 9.0-10.4. The established values of isoelectric points correspond with those found in the li

Název v anglickém jazyce

CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices

Popis výsledku anglicky

The effect of antibiotics on the microbial cells and concentration of antibiotics in the human body is essential for the effective use of antimicrobial therapy. The capillary isoelectric focusing is a suitable technique for the separation and the detection of bacteria, and amphoteric substances from nature. However, the determination of isoelectric points of ampholytic antibiotics by conventional techniques is time consuming. For this reason, capillary isoelectric focusing seems to be appropriate as a simple and reliable way for establishing them. The separation conditions for the capillary isoelectric focusing of selected ampholytic antibiotics with known isoelectric points and pK (a)s, ampicillin (pI 4.9), ciprofloxacin (pI 7.4), ofloxacin (pI 7.1),tetracycline (pI 5.4), tigecycline (pI 9.7), and vancomycin (pI 8.1), were found and optimized in the suitable pH ranges pH 2.0-5.3, 2.0-9.6, and 9.0-10.4. The established values of isoelectric points correspond with those found in the li

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

406

Číslo periodika v rámci svazku

25

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

6285-6296

Kód UT WoS článku

000342224300020

EID výsledku v databázi Scopus

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