HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065053" target="_blank" >RIV/00159816:_____/16:00065053 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/16:00088547 RIV/00216305:26230/16:PU122827

Výsledek na webu

<a href="http://dx.doi.org/10.1093/nar/gkw416" target="_blank" >http://dx.doi.org/10.1093/nar/gkw416</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkw416" target="_blank" >10.1093/nar/gkw416</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering

Popis výsledku v původním jazyce

Nucleic Acids Research, 2016 1 doi: 10.1093/nar/gkw416 HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering Jaroslav Bendl 1,2,3, + , Jan Stourac 1, + , Eva Sebestova 1 , Ondrej Vavra 1 , Milos Musil 1,2 , Jan Brezovsky 1,3,* and Jiri Damborsky 1,3,* 1 Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Masaryk University, 625 00 Brno, Czech Republic, 2 Department of Information Systems, Faculty of Information Technology, Brno University of Technology, 612 66 Brno, Czech Republic and 3 International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic Received February 21, 2016; Revised April 22, 2016; Accepted May 03, 2016 ABSTRACT HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart li- braries for engineering proteins' stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evo- lutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four dif- ferent protein engineering strategies and optimizing the resulting library's size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein's structure and visualized with a JSmol ap- plet. HotSpot Wizard lists annotated residues suit- able for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive an- notations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries.

Název v anglickém jazyce

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering

Popis výsledku anglicky

Nucleic Acids Research, 2016 1 doi: 10.1093/nar/gkw416 HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering Jaroslav Bendl 1,2,3, + , Jan Stourac 1, + , Eva Sebestova 1 , Ondrej Vavra 1 , Milos Musil 1,2 , Jan Brezovsky 1,3,* and Jiri Damborsky 1,3,* 1 Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Masaryk University, 625 00 Brno, Czech Republic, 2 Department of Information Systems, Faculty of Information Technology, Brno University of Technology, 612 66 Brno, Czech Republic and 3 International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic Received February 21, 2016; Revised April 22, 2016; Accepted May 03, 2016 ABSTRACT HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart li- braries for engineering proteins' stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evo- lutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four dif- ferent protein engineering strategies and optimizing the resulting library's size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein's structure and visualized with a JSmol ap- plet. HotSpot Wizard lists annotated residues suit- able for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive an- notations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

W1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

"W479"-"W487"

Kód UT WoS článku

000379786800079

EID výsledku v databázi Scopus

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