Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067590" target="_blank" >RIV/00159816:_____/17:00067590 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/17:00095459 RIV/68081707:_____/17:00506873

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0188772" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0188772</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0188772" target="_blank" >10.1371/journal.pone.0188772</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

Popis výsledku v původním jazyce

The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchro-nized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.

Název v anglickém jazyce

Comparative cell cycle transcriptomics reveals synchronization of developmental transcription factor networks in cancer cells

Popis výsledku anglicky

The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchro-nized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

24

Strana od-do

"e0188772"

Kód UT WoS článku

000417648600013

EID výsledku v databázi Scopus

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