Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00068657" target="_blank" >RIV/00159816:_____/18:00068657 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/18:00492427 RIV/00216224:14310/18:00106565 RIV/00209805:_____/18:00078101

Výsledek na webu

<a href="http://dx.doi.org/10.1038/bjc.2017.497" target="_blank" >http://dx.doi.org/10.1038/bjc.2017.497</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/bjc.2017.497" target="_blank" >10.1038/bjc.2017.497</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer

Popis výsledku v původním jazyce

Background: The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods: We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial-mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results: We found that surface CD9, CD29, CD49c, and integrin beta 5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions: We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial-mesenchymal plasticity in breast cancer.

Název v anglickém jazyce

Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer

Popis výsledku anglicky

Background: The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods: We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial-mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results: We found that surface CD9, CD29, CD49c, and integrin beta 5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions: We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial-mesenchymal plasticity in breast cancer.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

British journal of cancer

ISSN

0007-0920

e-ISSN

—

Svazek periodika

118

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

813-819

Kód UT WoS článku

000427945800020

EID výsledku v databázi Scopus

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