Interleukin-4 induces a CD44(high)/CD49b(high) PC3 subpopulation with tumor-initiating characteristics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00068674" target="_blank" >RIV/00159816:_____/18:00068674 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.26607" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.26607</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcb.26607" target="_blank" >10.1002/jcb.26607</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interleukin-4 induces a CD44(high)/CD49b(high) PC3 subpopulation with tumor-initiating characteristics

Popis výsledku v původním jazyce

Pro- and anti-inflammatory cytokines may influence proliferation, migration, invasion, and other cellular events of prostate cancer (PCa) cells. The hyaluronan receptor CD44, which is regulated by Interleukin (IL)-4, is a prostate basal cell marker. CD44(high)/CD49b(high) expressing cells have been demonstrated to have tumor-initiating characteristics. Here, we aimed to analyze the effects of long-term IL-4 treatment on CD44/CD49b expression, migration, proliferation, and clonogenic potential of basal-like PCa cells. To this end PC3 cells were treated over 30 passages with 5ng/mL IL-4 (PC3-IL4) resulting in an increased population of CD44(high) expressing cells. This was concurrent with a clonal outgrowth of cuboid-shaped cells, with increased size and light absorbance properties. Flow cytometry revealed that the PC3-IL4 CD44(high) expressing subpopulation corresponds to the CD49b(high) population. Isolation of the PC3-IL4 CD44(high)/CD49b(high) subpopulation via fluorescence-associated cell sorting showed increased migrative, proliferative, and clonogenic potential compared to the CD44(low)/CD49b(low) subpopulation. In conclusion, IL-4 increases a PC3 subpopulation with tumor-initiating characteristics. Thus, IL-4, similar to other cytokines may be a regulator of tumor-initiation and hence, may present a suitable therapy target in combination with current treatment options.

Název v anglickém jazyce

Interleukin-4 induces a CD44(high)/CD49b(high) PC3 subpopulation with tumor-initiating characteristics

Popis výsledku anglicky

Pro- and anti-inflammatory cytokines may influence proliferation, migration, invasion, and other cellular events of prostate cancer (PCa) cells. The hyaluronan receptor CD44, which is regulated by Interleukin (IL)-4, is a prostate basal cell marker. CD44(high)/CD49b(high) expressing cells have been demonstrated to have tumor-initiating characteristics. Here, we aimed to analyze the effects of long-term IL-4 treatment on CD44/CD49b expression, migration, proliferation, and clonogenic potential of basal-like PCa cells. To this end PC3 cells were treated over 30 passages with 5ng/mL IL-4 (PC3-IL4) resulting in an increased population of CD44(high) expressing cells. This was concurrent with a clonal outgrowth of cuboid-shaped cells, with increased size and light absorbance properties. Flow cytometry revealed that the PC3-IL4 CD44(high) expressing subpopulation corresponds to the CD49b(high) population. Isolation of the PC3-IL4 CD44(high)/CD49b(high) subpopulation via fluorescence-associated cell sorting showed increased migrative, proliferative, and clonogenic potential compared to the CD44(low)/CD49b(low) subpopulation. In conclusion, IL-4 increases a PC3 subpopulation with tumor-initiating characteristics. Thus, IL-4, similar to other cytokines may be a regulator of tumor-initiation and hence, may present a suitable therapy target in combination with current treatment options.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF CELLULAR BIOCHEMISTRY

ISSN

0730-2312

e-ISSN

—

Svazek periodika

119

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

4103-4112

Kód UT WoS článku

000428417900030

EID výsledku v databázi Scopus

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