HUMAN STEM CELL-DERIVED TISSUE ORGANOIDS AS A POTENTIAL TOOL FOR DRUG DELIVERY TESTING

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00070386" target="_blank" >RIV/00159816:_____/18:00070386 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HUMAN STEM CELL-DERIVED TISSUE ORGANOIDS AS A POTENTIAL TOOL FOR DRUG DELIVERY TESTING

Popis výsledku v původním jazyce

Advancement in organoid cultures from human pluripotent stem cells allows studying complexity of tissue specific interactions in vitro. This organ in a chip approach provides an important tool for translational research. Successful development of nanoparticles and nanostructures for clinical use has increased the need for more reliable and high-throughput experimental models beyond conventional cell culture. Human induced pluripotent stem cells (iPSCs) can be differentiated to multiple cell types including tissue-like organoids. Here, we study the interaction of nanoparticles with differentiated tissue cells and analyse their effects on various organoids in vitro. Human tissue organoids are differentiated from iPSCs by growth factor and by manipulation of various developmental signal pathways with inhibitors. The 3-dimentional structures of the bodies, with structural features similar to the appropriate human organ/tissue are obtained by embedding in a matrix. In order to test organoid as a model for interaction with nanoparticles, the organoids are injected with nano-sized particulate triggers such as glucans. The various tissue models are compared for their nanoparticle-induced stimulation by screening for inflammatory cytokines. Described model will allow studying human tissue interaction with nanoparticles, which for example are used in drug delivery.

Název v anglickém jazyce

HUMAN STEM CELL-DERIVED TISSUE ORGANOIDS AS A POTENTIAL TOOL FOR DRUG DELIVERY TESTING

Popis výsledku anglicky

Advancement in organoid cultures from human pluripotent stem cells allows studying complexity of tissue specific interactions in vitro. This organ in a chip approach provides an important tool for translational research. Successful development of nanoparticles and nanostructures for clinical use has increased the need for more reliable and high-throughput experimental models beyond conventional cell culture. Human induced pluripotent stem cells (iPSCs) can be differentiated to multiple cell types including tissue-like organoids. Here, we study the interaction of nanoparticles with differentiated tissue cells and analyse their effects on various organoids in vitro. Human tissue organoids are differentiated from iPSCs by growth factor and by manipulation of various developmental signal pathways with inhibitors. The 3-dimentional structures of the bodies, with structural features similar to the appropriate human organ/tissue are obtained by embedding in a matrix. In order to test organoid as a model for interaction with nanoparticles, the organoids are injected with nano-sized particulate triggers such as glucans. The various tissue models are compared for their nanoparticle-induced stimulation by screening for inflammatory cytokines. Described model will allow studying human tissue interaction with nanoparticles, which for example are used in drug delivery.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

<a href="/cs/project/LQ1605" target="_blank" >LQ1605: Translační medicína</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

9TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH &amp; APPLICATION (NANOCON 2017)

ISBN

978-80-87294-81-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

4

Strana od-do

589-592

Název nakladatele

TANGER LTD

Místo vydání

SLEZSKA

Místo konání akce

Brno

Datum konání akce

18. 10. 2017

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000452823300097