HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F20%3A00072959" target="_blank" >RIV/00159816:_____/20:00072959 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/20:00115466

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S1570023219305598?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1570023219305598?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jchromb.2019.121942" target="_blank" >10.1016/j.jchromb.2019.121942</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster

Popis výsledku v původním jazyce

A simple, sensitive HPLC-MS/MS method was developed and validated for the determination of lidocaine in skin and plasma of rats. The methods were established and validated assessing lower limit of quantitation (LLOQ), linearity, intra and inter-day precision and accuracy, selectivity, recovery and matrix effect. Chromatography was done on a Gemini column embedded with C18 stationary phase (50 mm x 2.0 mm, 5 mu m particle size), using a gradient with mobile phases consisting of 0.1% HCOOH in bidistilled water and 0.1% HCOOH in acetonitrile. The mass spectrometer worked with electrospray ionization in positive ion mode and selected reaction monitoring, using target ions m/z 235.10 for lidocaine and m/z 245.10 for lidocaine-d10, used as internal standard. Results: The linearity of the method was in the ranges of lidocaine concentrations 10.0-200.0 ng/mL for skin homogenate (accuracy 94.1-105.5%; R-2 &gt;= 0.998) and 0.025-2 ng/mL for plasma (accuracy 96.2-104.8%; R-2 &gt;= 0.996). The intra- and inter-day precision and accuracy determined on three quality control samples (20, 75 and 170 ng/mL for skin and 0.075, 0.4 and 1.5 ng/mL for plasma) were &lt;= 4.2% and 103.8-108.2% for skin and &lt;= 12.4% and 95.5-101.4% for plasma. The LLOQ was 10 ng/mL in skin homogenate and 0.025 ng/mL in plasma. The applicability of the method was demonstrated by measuring lidocaine in skin and plasma after exposure to medicated patches containing 5% lidocaine.

Název v anglickém jazyce

HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster

Popis výsledku anglicky

A simple, sensitive HPLC-MS/MS method was developed and validated for the determination of lidocaine in skin and plasma of rats. The methods were established and validated assessing lower limit of quantitation (LLOQ), linearity, intra and inter-day precision and accuracy, selectivity, recovery and matrix effect. Chromatography was done on a Gemini column embedded with C18 stationary phase (50 mm x 2.0 mm, 5 mu m particle size), using a gradient with mobile phases consisting of 0.1% HCOOH in bidistilled water and 0.1% HCOOH in acetonitrile. The mass spectrometer worked with electrospray ionization in positive ion mode and selected reaction monitoring, using target ions m/z 235.10 for lidocaine and m/z 245.10 for lidocaine-d10, used as internal standard. Results: The linearity of the method was in the ranges of lidocaine concentrations 10.0-200.0 ng/mL for skin homogenate (accuracy 94.1-105.5%; R-2 &gt;= 0.998) and 0.025-2 ng/mL for plasma (accuracy 96.2-104.8%; R-2 &gt;= 0.996). The intra- and inter-day precision and accuracy determined on three quality control samples (20, 75 and 170 ng/mL for skin and 0.075, 0.4 and 1.5 ng/mL for plasma) were &lt;= 4.2% and 103.8-108.2% for skin and &lt;= 12.4% and 95.5-101.4% for plasma. The LLOQ was 10 ng/mL in skin homogenate and 0.025 ng/mL in plasma. The applicability of the method was demonstrated by measuring lidocaine in skin and plasma after exposure to medicated patches containing 5% lidocaine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10609 - Biochemical research methods

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B : Analytical technologies in the biomedical and life sciences

ISSN

1570-0232

e-ISSN

—

Svazek periodika

1138

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000510111600002

EID výsledku v databázi Scopus

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