The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F22%3A00077761" target="_blank" >RIV/00159816:_____/22:00077761 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/22:00128839

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2589004222017114?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2589004222017114?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.isci.2022.105439" target="_blank" >10.1016/j.isci.2022.105439</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination

Popis výsledku v původním jazyce

During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function is regulated not only by several accessory proteins but also by bivalent ions. Here, we show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop. Based on structural studies, we have generated mutants of two highly conserved amino acids - E162 and D317 - in human DMC1, which are deficient in calcium regulation. In vivo studies of their yeast homologues further showed that they exhibit severe defects in meiosis, thus emphasizing the importance of calcium ions in the regulation of DMC1 function and meiotic recombination.

Název v anglickém jazyce

The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination

Popis výsledku anglicky

During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function is regulated not only by several accessory proteins but also by bivalent ions. Here, we show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop. Based on structural studies, we have generated mutants of two highly conserved amino acids - E162 and D317 - in human DMC1, which are deficient in calcium regulation. In vivo studies of their yeast homologues further showed that they exhibit severe defects in meiosis, thus emphasizing the importance of calcium ions in the regulation of DMC1 function and meiotic recombination.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10700 - Other natural sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ISCIENCE

ISSN

2589-0042

e-ISSN

2589-0042

Svazek periodika

25

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

27

Strana od-do

nestrankovano

Kód UT WoS článku

000923030100007

EID výsledku v databázi Scopus

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