Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F24%3A00081594" target="_blank" >RIV/00159816:_____/24:00081594 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/24:00135422

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0926337323010470" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0926337323010470</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apcatb.2023.123404" target="_blank" >10.1016/j.apcatb.2023.123404</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

Popis výsledku v původním jazyce

Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material properties and low cost. However, PET contributes significantly to the growing problem of plastic waste pollution. Enzymatic PET recycling has emerged as a promising alternative to conventional mechanical and chemical recycling methods. While many PET hydrolases - belonging to the alpha/beta-hydrolase fold superfamily - have been discovered, the wild -type enzymes obtained from natural sources are not optimal for industrial conditions and need to be optimized through rational design or directed evolution to improve their efficiency and stability. This Perspective sum-marizes case studies of engineered PET hydrolases and proposes a workflow that tightly integrates a variety of in silico and high-throughput approaches for biochemical and structural characterization to accelerate the discovery of PET-degrading enzymes, also with novel structural scaffolds. These biocatalysts could be candidates for developing further innovative plastic recycling techniques.

Název v anglickém jazyce

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

Popis výsledku anglicky

Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material properties and low cost. However, PET contributes significantly to the growing problem of plastic waste pollution. Enzymatic PET recycling has emerged as a promising alternative to conventional mechanical and chemical recycling methods. While many PET hydrolases - belonging to the alpha/beta-hydrolase fold superfamily - have been discovered, the wild -type enzymes obtained from natural sources are not optimal for industrial conditions and need to be optimized through rational design or directed evolution to improve their efficiency and stability. This Perspective sum-marizes case studies of engineered PET hydrolases and proposes a workflow that tightly integrates a variety of in silico and high-throughput approaches for biochemical and structural characterization to accelerate the discovery of PET-degrading enzymes, also with novel structural scaffolds. These biocatalysts could be candidates for developing further innovative plastic recycling techniques.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

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Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

APPLIED CATALYSIS B-ENVIRONMENTAL

ISSN

0926-3373

e-ISSN

1873-3883

Svazek periodika

342

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

16

Strana od-do

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Kód UT WoS článku

001110359200001

EID výsledku v databázi Scopus

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