A Simple Protocol for Visualization of RNA–Protein Complexes by Atomic Force Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00177016%3A_____%2F25%3AN0000136" target="_blank" >RIV/00177016:_____/25:N0000136 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/25:00140473

Výsledek na webu

<a href="https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70084" target="_blank" >https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70084</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A Simple Protocol for Visualization of RNA–Protein Complexes by Atomic Force Microscopy

Popis výsledku v původním jazyce

Atomic force microscopy (AFM) has recently received increasing interest in molecular biology. This technique allows quick and reliable detection of biomolecules. However, studying RNA-protein complexes using AFM poses significant challenges. Here, we describe a simple and reliable method to visualize positively charged proteins bound to RNA that does not require metallic cations. This method allowed us to effectively detect and visualize Staufen-RNA complexes by height or logarithmic stiffness. The study of the mechanical properties is particularly important in the case of protein-coated RNA complexes, where RNA cannot be detected by height channel. In any case, it is necessary to compare AFM data with the data derived from other techniques like nuclear magnetic resonance, X-ray crystallography, cryogenic electron microscopy, and small-angle X-ray scattering. (c) 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Preparation and visualization of RNA-protein complex

Název v anglickém jazyce

A Simple Protocol for Visualization of RNA–Protein Complexes by Atomic Force Microscopy

Popis výsledku anglicky

Atomic force microscopy (AFM) has recently received increasing interest in molecular biology. This technique allows quick and reliable detection of biomolecules. However, studying RNA-protein complexes using AFM poses significant challenges. Here, we describe a simple and reliable method to visualize positively charged proteins bound to RNA that does not require metallic cations. This method allowed us to effectively detect and visualize Staufen-RNA complexes by height or logarithmic stiffness. The study of the mechanical properties is particularly important in the case of protein-coated RNA complexes, where RNA cannot be detected by height channel. In any case, it is necessary to compare AFM data with the data derived from other techniques like nuclear magnetic resonance, X-ray crystallography, cryogenic electron microscopy, and small-angle X-ray scattering. (c) 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Preparation and visualization of RNA-protein complex

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF18_046%2F0015974" target="_blank" >EF18_046/0015974: Modernizace České infrastruktury pro integrativní strukturní biologii</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Protocols

ISSN

2691-1299

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

001391965600001

EID výsledku v databázi Scopus

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