Susceptibility of rat non-alcoholic fatty liver to the acute toxic effect of acetaminophen

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F12%3A10125015" target="_blank" >RIV/00179906:_____/12:10125015 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/12:10125015

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1440-1746.2011.06807.x/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1440-1746.2011.06807.x/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1440-1746.2011.06807.x" target="_blank" >10.1111/j.1440-1746.2011.06807.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Susceptibility of rat non-alcoholic fatty liver to the acute toxic effect of acetaminophen

Popis výsledku v původním jazyce

Acetaminophen overdose is the most frequent cause of acute liver failure. Non-alcoholic fatty liver disease is the most common chronic condition of the liver. The aim was to assess whether non-alcoholic steatosis sensitizes rat liver to acute toxic effect of acetaminophen. Male Sprague-Dawley rats were fed a standard diet (ST-1, 10 % kcal fat) and high-fat gelled diet (HFGD, 71% kcal fat) for 6 weeks and then acetaminophen was applied in a single dose (1 g/kg body weight). Animals were killed 24, 48 and72 h after acetaminophen administration. Serum biochemistry, activities of mitochondrial complexes, hepatic malondialdehyde, reduced and oxidized glutathione, triacylglycerol and cholesterol contents, and concentrations of serum and liver cytokines (TNF-alpha, TGF-beta1) were measured and histopathological samples were prepared. The degree of liver inflammation and hepatocellular necrosis were significantly higher in HFGD fed animals after acetaminophen administration. Serum markers of

Název v anglickém jazyce

Susceptibility of rat non-alcoholic fatty liver to the acute toxic effect of acetaminophen

Popis výsledku anglicky

Acetaminophen overdose is the most frequent cause of acute liver failure. Non-alcoholic fatty liver disease is the most common chronic condition of the liver. The aim was to assess whether non-alcoholic steatosis sensitizes rat liver to acute toxic effect of acetaminophen. Male Sprague-Dawley rats were fed a standard diet (ST-1, 10 % kcal fat) and high-fat gelled diet (HFGD, 71% kcal fat) for 6 weeks and then acetaminophen was applied in a single dose (1 g/kg body weight). Animals were killed 24, 48 and72 h after acetaminophen administration. Serum biochemistry, activities of mitochondrial complexes, hepatic malondialdehyde, reduced and oxidized glutathione, triacylglycerol and cholesterol contents, and concentrations of serum and liver cytokines (TNF-alpha, TGF-beta1) were measured and histopathological samples were prepared. The degree of liver inflammation and hepatocellular necrosis were significantly higher in HFGD fed animals after acetaminophen administration. Serum markers of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP305%2F08%2FP184" target="_blank" >GP305/08/P184: Jsou játra postižená nealkoholickým ztukovatěním (non-alcoholic fatty liver disease, NAFLD) citlivější vůči toxickému poškození?</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Gastroenterology and Hepatology

ISSN

0815-9319

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

323-330

Kód UT WoS článku

000299336400023

EID výsledku v databázi Scopus

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