Identification and functional screening of microRNAs highly deregulated in colorectal cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F12%3A10129317" target="_blank" >RIV/00179906:_____/12:10129317 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/12:00059697 RIV/00209805:_____/12:#0000340 RIV/00216208:11150/12:10129317

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1582-4934.2012.01579.x" target="_blank" >http://dx.doi.org/10.1111/j.1582-4934.2012.01579.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1582-4934.2012.01579.x" target="_blank" >10.1111/j.1582-4934.2012.01579.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and functional screening of microRNAs highly deregulated in colorectal cancer

Popis výsledku v původním jazyce

MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumour suppressors. We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoural tissues and identified 42 differentially expressed miRNAs. We chose miR-215, miR-375, miR-378, miR-422a and miR-135b for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro analyses. MiR-215, miR-375, miR-378 and miR-422a were significantly decreased, whereas miR-135b was increased in CRC tumour tissues. Levels of miR-215 and miR-422a correlated with clinical stage. MiR-135b was associated with higherpre-operative serum levels of CEA and CA19-9. In vitro analyses showed that ectopic expression of miR-215 decreases viability and migration, increases apoptosis and promotes cell cycle arrest in DLD-1 and HCT-116 colon cancer cell lines.

Název v anglickém jazyce

Identification and functional screening of microRNAs highly deregulated in colorectal cancer

Popis výsledku anglicky

MicroRNAs (miRNAs) constitute a robust regulatory network with post-transcriptional regulatory efficiency for almost one half of human coding genes, including oncogenes and tumour suppressors. We determined the expression profile of 667 miRNAs in colorectal cancer (CRC) tissues and paired non-tumoural tissues and identified 42 differentially expressed miRNAs. We chose miR-215, miR-375, miR-378, miR-422a and miR-135b for further validation on an independent cohort of 125 clinically characterized CRC patients and for in vitro analyses. MiR-215, miR-375, miR-378 and miR-422a were significantly decreased, whereas miR-135b was increased in CRC tumour tissues. Levels of miR-215 and miR-422a correlated with clinical stage. MiR-135b was associated with higherpre-operative serum levels of CEA and CA19-9. In vitro analyses showed that ectopic expression of miR-215 decreases viability and migration, increases apoptosis and promotes cell cycle arrest in DLD-1 and HCT-116 colon cancer cell lines.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cellular and Molecular Medicine

ISSN

1582-1838

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

RO - Rumunsko

Počet stran výsledku

12

Strana od-do

2655-2666

Kód UT WoS článku

000310555200010

EID výsledku v databázi Scopus

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