Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and alpha-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10145598" target="_blank" >RIV/00179906:_____/13:10145598 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/13:10145598

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300242/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300242/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300242" target="_blank" >10.1002/jssc.201300242</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and alpha-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography

Popis výsledku v původním jazyce

The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high-throughput assay methods in bioanalysis. It can be achieved either by ultra-high-performance liquid chromatography (UHPLC)using stationary phases with small particles (<2 micro m) at high back pressures or by using opposite direction - monolithic stationary phases with low back pressures. The application of new types of monolithic stationary phases for UHPLC is a novel idea combining these two different paths. The aim of this work was to test the recently introduced second-generation of monolithic column Chromolith (R) HighResolution for UHPLC analysis of liposoluble vitamins in comparison with core-shell and fully poroussub-2 micro m columns with different particle sizes, column lengths, and shapes. The separation efficiency, peak shape, resolution, time of analysis, consumption of mobile phase, and lifetime of columns were calculated and compared. The

Název v anglickém jazyce

Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and alpha-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography

Popis výsledku anglicky

The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high-throughput assay methods in bioanalysis. It can be achieved either by ultra-high-performance liquid chromatography (UHPLC)using stationary phases with small particles (<2 micro m) at high back pressures or by using opposite direction - monolithic stationary phases with low back pressures. The application of new types of monolithic stationary phases for UHPLC is a novel idea combining these two different paths. The aim of this work was to test the recently introduced second-generation of monolithic column Chromolith (R) HighResolution for UHPLC analysis of liposoluble vitamins in comparison with core-shell and fully poroussub-2 micro m columns with different particle sizes, column lengths, and shapes. The separation efficiency, peak shape, resolution, time of analysis, consumption of mobile phase, and lifetime of columns were calculated and compared. The

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Vybudování výzkumného týmu experimentální a aplikované biofarmacie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

36

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

2223-2230

Kód UT WoS článku

000321760600002

EID výsledku v databázi Scopus

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