Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10188841" target="_blank" >RIV/00179906:_____/13:10188841 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/13:10188841

Výsledek na webu

<a href="http://dx.doi.org/10.3109/00365548.2013.795656" target="_blank" >http://dx.doi.org/10.3109/00365548.2013.795656</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3109/00365548.2013.795656" target="_blank" >10.3109/00365548.2013.795656</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis

Popis výsledku v původním jazyce

Background: The aims of this work were to replace the obsolete PCR method for the laboratory diagnosis of the acute form of leptospirosis using the G1, G2 an B64 I, B64 II primers, and to improve the PCR detection time.Methods: We introduced a real-timePCR method for the detection of the gene encoding the surface lipoprotein LipL32 of pathogenic Leptospira into our laboratory diagnosis of the acute fom of leptospirosis. The positive and negative analytical specificities of the real-time PCR method wereboth equal to 100%; the detection limit was determined to be 1-5 genome copies/1 ml of liquid biological material.The method was further validated on 230 laboratory strains of leptospires. Results: All laboratory strains of pathogenic Leptospira were evaluated as LipL32-positive and all non-pathogenic strains as LipL32-negative. In addition, 455 biological materials (253 plasma, 121 urine, 72 cerebrospinal fluid (CSF), 7 bronchoalveolar lavage, and 2 sputum) from 295 patients with suspe

Název v anglickém jazyce

Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis

Popis výsledku anglicky

Background: The aims of this work were to replace the obsolete PCR method for the laboratory diagnosis of the acute form of leptospirosis using the G1, G2 an B64 I, B64 II primers, and to improve the PCR detection time.Methods: We introduced a real-timePCR method for the detection of the gene encoding the surface lipoprotein LipL32 of pathogenic Leptospira into our laboratory diagnosis of the acute fom of leptospirosis. The positive and negative analytical specificities of the real-time PCR method wereboth equal to 100%; the detection limit was determined to be 1-5 genome copies/1 ml of liquid biological material.The method was further validated on 230 laboratory strains of leptospires. Results: All laboratory strains of pathogenic Leptospira were evaluated as LipL32-positive and all non-pathogenic strains as LipL32-negative. In addition, 455 biological materials (253 plasma, 121 urine, 72 cerebrospinal fluid (CSF), 7 bronchoalveolar lavage, and 2 sputum) from 295 patients with suspe

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scandinavian Journal of Infectious Diseases

ISSN

0036-5548

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NO - Norské království

Počet stran výsledku

7

Strana od-do

593-599

Kód UT WoS článku

000321789300003

EID výsledku v databázi Scopus

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