DNA/BSA binding ability and genotoxic effect of mono- and binuclear copper (II) complexes containing a Schiff base derived from salicylaldehyde and D, L-glutamic acid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F17%3A10337623" target="_blank" >RIV/00179906:_____/17:10337623 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jorganchem.2016.11.007" target="_blank" >http://dx.doi.org/10.1016/j.jorganchem.2016.11.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jorganchem.2016.11.007" target="_blank" >10.1016/j.jorganchem.2016.11.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DNA/BSA binding ability and genotoxic effect of mono- and binuclear copper (II) complexes containing a Schiff base derived from salicylaldehyde and D, L-glutamic acid

Popis výsledku v původním jazyce

This study examined a two of Cu(II) complexes containing Schiff bases [Cu(salglu)Meim] (1) and [Cu(et)(2)(im)(4)][Cu-2(salglu)(2)(im)(2)] (2), where salglu = (N-salicylidene-D,L-glutamato)(2-) Meim = 2-methylimidazole, im = imidazole and et = ethanol. The interactions and binding characteristics of the complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were investigated using UV-Vis and fluorescence spectroscopy. The binding constants K were calculated from the UV-Vis spectroscopic titration results using the McGhee and Hippel equation, and were found to be K = 8.9 (for 1) and 3.1 x 10(3) M-1 (for 2) in the case of complex-DNA interactions, while Stern-Volmer quenching results were used to determine the values K = 2.74 x 10(5) and 2.54 x 10(6) M-1 (for 1 and 2, respectively) in the case of complex-BSA binding. DNA strand breaks in cellular DNA were investigated using single cell gel electrophoresis (comet assay). MCF-7 cancer cell lines and healthy HEK-293T cell lines were exposed to complexes 1 and 2 at a concentration range of 0.001-100 x 10(-6) M for 24 h. Complexes 1 and 2 induced significant DNA damage in MCF-7 cancer cell lines, while healthy HEK-293T cell lines were more resistant against their effect.

Název v anglickém jazyce

DNA/BSA binding ability and genotoxic effect of mono- and binuclear copper (II) complexes containing a Schiff base derived from salicylaldehyde and D, L-glutamic acid

Popis výsledku anglicky

This study examined a two of Cu(II) complexes containing Schiff bases [Cu(salglu)Meim] (1) and [Cu(et)(2)(im)(4)][Cu-2(salglu)(2)(im)(2)] (2), where salglu = (N-salicylidene-D,L-glutamato)(2-) Meim = 2-methylimidazole, im = imidazole and et = ethanol. The interactions and binding characteristics of the complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were investigated using UV-Vis and fluorescence spectroscopy. The binding constants K were calculated from the UV-Vis spectroscopic titration results using the McGhee and Hippel equation, and were found to be K = 8.9 (for 1) and 3.1 x 10(3) M-1 (for 2) in the case of complex-DNA interactions, while Stern-Volmer quenching results were used to determine the values K = 2.74 x 10(5) and 2.54 x 10(6) M-1 (for 1 and 2, respectively) in the case of complex-BSA binding. DNA strand breaks in cellular DNA were investigated using single cell gel electrophoresis (comet assay). MCF-7 cancer cell lines and healthy HEK-293T cell lines were exposed to complexes 1 and 2 at a concentration range of 0.001-100 x 10(-6) M for 24 h. Complexes 1 and 2 induced significant DNA damage in MCF-7 cancer cell lines, while healthy HEK-293T cell lines were more resistant against their effect.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Organometallic Chemistry

ISSN

0022-328X

e-ISSN

—

Svazek periodika

827

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

67-77

Kód UT WoS článku

000389610700008

EID výsledku v databázi Scopus

2-s2.0-84994651595