Antiangiogenic Human Monoclonal Antibody Ramucirumab Radiolabelling: In Vitro Evaluation on VEGFR2-positive Cell Lines
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F19%3A10397895" target="_blank" >RIV/00179906:_____/19:10397895 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11160/19:10397895 RIV/61989592:15110/19:73592331
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=sgoHllYj51" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=sgoHllYj51</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21873/anticanres.13170" target="_blank" >10.21873/anticanres.13170</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Antiangiogenic Human Monoclonal Antibody Ramucirumab Radiolabelling: In Vitro Evaluation on VEGFR2-positive Cell Lines
Popis výsledku v původním jazyce
Background/Aim: Radiolabelling of monoclonal antibodies (mAbs) could be beneficial in cancer diagnosis and therapy, however it may cause structural changes and consequently deteriorate their immunoreactivity. Materials and Methods: The therapeutic mAb ramucirumab (RAM) was technetium-99m labelled using either a direct or an indirect method with the use of two bifunctional chelating agents (HYNIC, DTPA). The radiochemical purity was assessed using instant thin-layer chromatography (ITLC) and high-performance liquid chromatography (HPLC) technique. The affinity of radiolabelled RAM was tested on human cancer cell lines. Results: The radiolabelling provided the following stable compounds: [Tc-99m]RAM, [Tc-99m]HYNIC-RAM and [Tc-99m]DTPA-RAM. Their radiochemical purity was over 95%. All prepared radiopharmaceuticals showed moderate affinity to the targeted receptor, in vitro. However, their affinity was one order lower compared to that of the natural mAb. Moreover, directly and DTPA-radiolabelled RAM demonstrated less favourable binding kinetics. Conclusion: Radiolabelling negatively affected the affinity of RAM to its targeted receptor.
Název v anglickém jazyce
Antiangiogenic Human Monoclonal Antibody Ramucirumab Radiolabelling: In Vitro Evaluation on VEGFR2-positive Cell Lines
Popis výsledku anglicky
Background/Aim: Radiolabelling of monoclonal antibodies (mAbs) could be beneficial in cancer diagnosis and therapy, however it may cause structural changes and consequently deteriorate their immunoreactivity. Materials and Methods: The therapeutic mAb ramucirumab (RAM) was technetium-99m labelled using either a direct or an indirect method with the use of two bifunctional chelating agents (HYNIC, DTPA). The radiochemical purity was assessed using instant thin-layer chromatography (ITLC) and high-performance liquid chromatography (HPLC) technique. The affinity of radiolabelled RAM was tested on human cancer cell lines. Results: The radiolabelling provided the following stable compounds: [Tc-99m]RAM, [Tc-99m]HYNIC-RAM and [Tc-99m]DTPA-RAM. Their radiochemical purity was over 95%. All prepared radiopharmaceuticals showed moderate affinity to the targeted receptor, in vitro. However, their affinity was one order lower compared to that of the natural mAb. Moreover, directly and DTPA-radiolabelled RAM demonstrated less favourable binding kinetics. Conclusion: Radiolabelling negatively affected the affinity of RAM to its targeted receptor.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30104 - Pharmacology and pharmacy
Návaznosti výsledku
Projekt
<a href="/cs/project/LO1304" target="_blank" >LO1304: Podpora udržitelnosti Ústavu molekulární a translační medicíny</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Anticancer Research
ISSN
0250-7005
e-ISSN
—
Svazek periodika
39
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
GR - Řecká republika
Počet stran výsledku
10
Strana od-do
735-744
Kód UT WoS článku
000457451200027
EID výsledku v databázi Scopus
2-s2.0-85060983263