Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10466207" target="_blank" >RIV/00179906:_____/23:10466207 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13440/23:43897743 RIV/00216208:11150/23:10466207

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Nj4N7HCFJ5" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Nj4N7HCFJ5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12865-023-00544-5" target="_blank" >10.1186/s12865-023-00544-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome

Popis výsledku v původním jazyce

Background: Patients with Sjogren&apos;s syndrome, like other patients with autoimmune disorders, display dysregulation in the function of their immune system. Fas and Fas Ligand (FasL) are among the dysregulated proteins. Methods: We studied Fas and FasL on IL-2R alpha(+) cells and in serum of patients with Sjogren&apos;s syndrome (n = 16) and healthy individuals (n = 16); both from same ethnic and geographical background. We used flow cytometry and enzyme-linked immunosorbent for this purpose. We also measured the expression of Bcl-2 and Bax by reverse transcription quantitative real-time PCR (RT-qPCR) and percentage of apoptotic and dead cells using Annexin V and 7-AAD staining in lymphocytes. Results: FasL was increased in patients&apos; T and B cells while Fas was increased in patients&apos; monocytes, T and B cells. No signs of increased apoptosis were found. sFas and sFasL in patients&apos; serum were increased, although the increase in sFasL was not significant. We suspect an effect of non-steroidal anti-inflammatory therapy on B cells, explaining the decrease of the percentage Fas(+) B cells found within our samples. In healthy individuals, there was a noticeable pattern in the expression of FasL which mutually correlated to populations of mononuclear cells; this correlation was absent in the patients with Sjogren&apos;s syndrome. Conclusion: sMononuclear cells expressing IL-2R alpha(+) had upregulated Fas in Sjogren&apos;s syndrome. However, the rate of apoptosis based on Annexin V staining and the Bcl-2/Bax expression was not observed in mononuclear cells. We suspect a functional role of abnormal levels of Fas and FasL which has not been cleared yet.

Název v anglickém jazyce

Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome

Popis výsledku anglicky

Background: Patients with Sjogren&apos;s syndrome, like other patients with autoimmune disorders, display dysregulation in the function of their immune system. Fas and Fas Ligand (FasL) are among the dysregulated proteins. Methods: We studied Fas and FasL on IL-2R alpha(+) cells and in serum of patients with Sjogren&apos;s syndrome (n = 16) and healthy individuals (n = 16); both from same ethnic and geographical background. We used flow cytometry and enzyme-linked immunosorbent for this purpose. We also measured the expression of Bcl-2 and Bax by reverse transcription quantitative real-time PCR (RT-qPCR) and percentage of apoptotic and dead cells using Annexin V and 7-AAD staining in lymphocytes. Results: FasL was increased in patients&apos; T and B cells while Fas was increased in patients&apos; monocytes, T and B cells. No signs of increased apoptosis were found. sFas and sFasL in patients&apos; serum were increased, although the increase in sFasL was not significant. We suspect an effect of non-steroidal anti-inflammatory therapy on B cells, explaining the decrease of the percentage Fas(+) B cells found within our samples. In healthy individuals, there was a noticeable pattern in the expression of FasL which mutually correlated to populations of mononuclear cells; this correlation was absent in the patients with Sjogren&apos;s syndrome. Conclusion: sMononuclear cells expressing IL-2R alpha(+) had upregulated Fas in Sjogren&apos;s syndrome. However, the rate of apoptosis based on Annexin V staining and the Bcl-2/Bax expression was not observed in mononuclear cells. We suspect a functional role of abnormal levels of Fas and FasL which has not been cleared yet.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30208 - Dentistry, oral surgery and medicine

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Immunology

ISSN

1471-2172

e-ISSN

1471-2172

Svazek periodika

24

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

12

Kód UT WoS článku

001011253300001

EID výsledku v databázi Scopus

2-s2.0-85162754222