Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F24%3A10484168" target="_blank" >RIV/00179906:_____/24:10484168 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62690094:18470/24:50021583

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=.0r1LQQaGl" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=.0r1LQQaGl</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbi.2024.111138" target="_blank" >10.1016/j.cbi.2024.111138</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats

Popis výsledku v původním jazyce

Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators- asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD 50 /kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes &apos; application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups ( p &lt; 0.001). The activity of CAT was significantly elevated in the obidoximetreated group ( p &lt; 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels ( p &lt; 0.01, p &lt; 0.001). Interestingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels ( p &lt; 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats.

Název v anglickém jazyce

Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats

Popis výsledku anglicky

Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators- asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD 50 /kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes &apos; application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups ( p &lt; 0.001). The activity of CAT was significantly elevated in the obidoximetreated group ( p &lt; 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels ( p &lt; 0.01, p &lt; 0.001). Interestingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels ( p &lt; 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemico-Biological Interactions

ISSN

0009-2797

e-ISSN

1872-7786

Svazek periodika

399

Číslo periodika v rámci svazku

AUG

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

111138

Kód UT WoS článku

001272683000001

EID výsledku v databázi Scopus

2-s2.0-85198293401