PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F03%3A%230000255" target="_blank" >RIV/00209775:_____/03:#0000255 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2003.007.19x/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2003.007.19x/pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia

Popis výsledku v původním jazyce

To assess the usefulness of polymerase chain reaction PCR) assays in the diagnosis of fungal infections in immunocompromised patients. A rapid and sensitive PCR-based assay for the detection and identification of fungal pathogens was designed and applicability of this method was investigated in a group of children with cancer and febrile neutropenia (FN). The ITS2 sequences and adjacent regions of 40 fungal pathogens were analyzed and primers for detection of all analyzed fungal species were designed. Amplification product lenght polymorphism (APLP) and restriction fragment lenght polymorphism (RFLP) generated genus- or species-specific patterns. The sensitivity of the method was approximately three cells of Candida albicans per 1 mL of blood. The results were available within 8 h after sample collection. The method was tested on 53 blood samples and one lung biopsy sample form 24 children with cancer and febrile neutropenia (FN). The PCR assay detected fungal DNA in 25 clinical sample

Název v anglickém jazyce

PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia

Popis výsledku anglicky

To assess the usefulness of polymerase chain reaction PCR) assays in the diagnosis of fungal infections in immunocompromised patients. A rapid and sensitive PCR-based assay for the detection and identification of fungal pathogens was designed and applicability of this method was investigated in a group of children with cancer and febrile neutropenia (FN). The ITS2 sequences and adjacent regions of 40 fungal pathogens were analyzed and primers for detection of all analyzed fungal species were designed. Amplification product lenght polymorphism (APLP) and restriction fragment lenght polymorphism (RFLP) generated genus- or species-specific patterns. The sensitivity of the method was approximately three cells of Candida albicans per 1 mL of blood. The results were available within 8 h after sample collection. The method was tested on 53 blood samples and one lung biopsy sample form 24 children with cancer and febrile neutropenia (FN). The PCR assay detected fungal DNA in 25 clinical sample

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/NM6870" target="_blank" >NM6870: Detekce příčin septických stavů po rozsáhlých chirurgických výkonech: srovnání hemokultivace a PCR</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical microbiology and infection

ISSN

1469-0691

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

1191-1202

Kód UT WoS článku

000187255800004

EID výsledku v databázi Scopus

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