Specific Modulation of p53 Binding to Supercoiled DNA with and without Consensus Sequence by Monoclonal Antibodies

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Specific Modulation of p53 Binding to Supercoiled DNA with and without Consensus Sequence by Monoclonal Antibodies

Popis výsledku v původním jazyce

The wild type human p53 protein binds preferentially to supercoiled DNA in vitro both in the presence and absence of p53 consensus sequence. The p53-DNA binding is decreased by physiological concentrations of zinc ions and at higher zinc concentrations the binding of p53 to sc DNA is completely inhibited. In contrast, oxidation of the protein with diamide resulted in partial inhibition of p53 - scDNA binding. The activation of p53 for sequence-specific binding within scDNA was also studied. C-terminal specific antibody Bp53-10 activated the sequence-specific binding of p53 to p53CON within pPGM1 scDNA but did not influence its binding to pBluescript scDNA.

Název v anglickém jazyce

Specific Modulation of p53 Binding to Supercoiled DNA with and without Consensus Sequence by Monoclonal Antibodies

Popis výsledku anglicky

The wild type human p53 protein binds preferentially to supercoiled DNA in vitro both in the presence and absence of p53 consensus sequence. The p53-DNA binding is decreased by physiological concentrations of zinc ions and at higher zinc concentrations the binding of p53 to sc DNA is completely inhibited. In contrast, oxidation of the protein with diamide resulted in partial inhibition of p53 - scDNA binding. The activation of p53 for sequence-specific binding within scDNA was also studied. C-terminal specific antibody Bp53-10 activated the sequence-specific binding of p53 to p53CON within pPGM1 scDNA but did not influence its binding to pBluescript scDNA.

Druh

D - Stať ve sborníku

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

10th International p53 Workshop

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

neuv.

Místo vydání

Monterey

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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