Intracellular distribution of the ?Np73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F13%3A%230000406" target="_blank" >RIV/00209805:_____/13:#0000406 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm" target="_blank" >http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Intracellular distribution of the ?Np73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Popis výsledku v původním jazyce

The p73 protein is a member of the p53 family of transcription factors that has two N-terminal isoforms: the TAp73 isoform is reported to have a tumor suppressor function, whereas the ?Np73 isoform likely has oncogenic potential. The expression of theseisoforms and the differences in their intracellular distribution have been described in many cancer types; however, little is known about the p73 isoforms in brain tumors. Our study is focused on the intracellular localization of ?Np73 in medulloblastomacell lines. Due to a lack of suitable anti-?Np73 antibodies, we developed two new rabbit polyclonal antibodies, ?Np73-26 and ?Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Bothof these new antibodies were subsequently used for analysis of the ?Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear loca

Název v anglickém jazyce

Intracellular distribution of the ?Np73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Popis výsledku anglicky

The p73 protein is a member of the p53 family of transcription factors that has two N-terminal isoforms: the TAp73 isoform is reported to have a tumor suppressor function, whereas the ?Np73 isoform likely has oncogenic potential. The expression of theseisoforms and the differences in their intracellular distribution have been described in many cancer types; however, little is known about the p73 isoforms in brain tumors. Our study is focused on the intracellular localization of ?Np73 in medulloblastomacell lines. Due to a lack of suitable anti-?Np73 antibodies, we developed two new rabbit polyclonal antibodies, ?Np73-26 and ?Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Bothof these new antibodies were subsequently used for analysis of the ?Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear loca

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Histology and Histopathology

ISSN

0213-3911

e-ISSN

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Svazek periodika

28

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

ES - Španělské království

Počet stran výsledku

12

Strana od-do

913-924

Kód UT WoS článku

000320710900011

EID výsledku v databázi Scopus

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