A miniaturized device for bioluminescence analysis of caspase-3/7 activity in a single apoptotic cell

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F14%3A%230000552" target="_blank" >RIV/00209805:_____/14:#0000552 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/14:00437556 RIV/62157124:16170/14:43872987

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs00216-014-7949-7" target="_blank" >http://link.springer.com/article/10.1007%2Fs00216-014-7949-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-014-7949-7" target="_blank" >10.1007/s00216-014-7949-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A miniaturized device for bioluminescence analysis of caspase-3/7 activity in a single apoptotic cell

Popis výsledku v původním jazyce

Caspases are key enzymes activated during the apoptotic machinery. Apoptosis as a way of programmed cell death becomes deregulated in some pathologies including cancer transformations, neurodegenerative, or autoimmune diseases. Most of the methods available for the detection of apoptosis and caspases provide qualitative information only or quantification data as an average from cell populations or cell lysates. Several reports point to the importance of more accurate single-cell analyses in biomedical studies due to heterogeneity at tissue as well as cell level. To meet these requirements, we developed a miniaturized device enabling detection and quantification of active caspase-3/7 in individual cells at a femtogram level (10-15 g). The active caspase-3/7 detection protocol is based on the bioluminescence chemistry commercially available as a Caspase-GloTM 3/7 reagent developed by Promega. As a model, we used human stem cells treated by camptothecin to induce apoptosis. Individual apo

Název v anglickém jazyce

A miniaturized device for bioluminescence analysis of caspase-3/7 activity in a single apoptotic cell

Popis výsledku anglicky

Caspases are key enzymes activated during the apoptotic machinery. Apoptosis as a way of programmed cell death becomes deregulated in some pathologies including cancer transformations, neurodegenerative, or autoimmune diseases. Most of the methods available for the detection of apoptosis and caspases provide qualitative information only or quantification data as an average from cell populations or cell lysates. Several reports point to the importance of more accurate single-cell analyses in biomedical studies due to heterogeneity at tissue as well as cell level. To meet these requirements, we developed a miniaturized device enabling detection and quantification of active caspase-3/7 in individual cells at a femtogram level (10-15 g). The active caspase-3/7 detection protocol is based on the bioluminescence chemistry commercially available as a Caspase-GloTM 3/7 reagent developed by Promega. As a model, we used human stem cells treated by camptothecin to induce apoptosis. Individual apo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

406

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

5389-5394

Kód UT WoS článku

000340877200017

EID výsledku v databázi Scopus

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