Regulation of AGR2 expression via 3’UTR shortening

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F17%3A00077875" target="_blank" >RIV/00209805:_____/17:00077875 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0014482717302227?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014482717302227?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.yexcr.2017.04.011" target="_blank" >10.1016/j.yexcr.2017.04.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Regulation of AGR2 expression via 3’UTR shortening

Popis výsledku v původním jazyce

One recently discussed general mechanism affecting gene expression is 3&apos;-untranslated region (3&apos;UTR) length. Events such as shortening, translocation or loss of 3&apos;UTRs are observed within oncogenes and are proposed to associate with increased expression. Thus, increased efforts are being made to understand constitutive and differential transcript 3&apos;end formation. Investigation of AGR2 mRNA revealed a direct impact of its 3&apos;UTR length on AGR2 expression. In silico analyses identified several regulatory sequences within the distal part of AGR2 mRNA that may regulate 3&apos;UTR length and associated protein levels. Short 3&apos;UTRs were observed in a panel of AGR2-positive cancer cell lines and in human breast cancer specimens, in which more extensive 3&apos;UTR shortening correlated with increased AGR2 protein levels. AGR2 is an important member of PI3K/AKT signalling pathway, which along with the proposed involvement of mTOR in the regulation of alternative polyadenylation, prompted us to study the role of mTOR in relation to AGR2 mRNA 3&apos;UTR shortening. A direct impact of mTOR signalling on AGR2 3&apos;UTR shortening associated with increased protein synthesis was found, which led to the identification of a novel molecular mechanism involved in upregulation of AGR2 levels in mTOR-activated cells via modulating the 3&apos;UTR length of AGR2 mRNA.

Název v anglickém jazyce

Regulation of AGR2 expression via 3’UTR shortening

Popis výsledku anglicky

One recently discussed general mechanism affecting gene expression is 3&apos;-untranslated region (3&apos;UTR) length. Events such as shortening, translocation or loss of 3&apos;UTRs are observed within oncogenes and are proposed to associate with increased expression. Thus, increased efforts are being made to understand constitutive and differential transcript 3&apos;end formation. Investigation of AGR2 mRNA revealed a direct impact of its 3&apos;UTR length on AGR2 expression. In silico analyses identified several regulatory sequences within the distal part of AGR2 mRNA that may regulate 3&apos;UTR length and associated protein levels. Short 3&apos;UTRs were observed in a panel of AGR2-positive cancer cell lines and in human breast cancer specimens, in which more extensive 3&apos;UTR shortening correlated with increased AGR2 protein levels. AGR2 is an important member of PI3K/AKT signalling pathway, which along with the proposed involvement of mTOR in the regulation of alternative polyadenylation, prompted us to study the role of mTOR in relation to AGR2 mRNA 3&apos;UTR shortening. A direct impact of mTOR signalling on AGR2 3&apos;UTR shortening associated with increased protein synthesis was found, which led to the identification of a novel molecular mechanism involved in upregulation of AGR2 levels in mTOR-activated cells via modulating the 3&apos;UTR length of AGR2 mRNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental cell research

ISSN

0014-4827

e-ISSN

—

Svazek periodika

356

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

40-47

Kód UT WoS článku

000402776700005

EID výsledku v databázi Scopus

2-s2.0-85017408948