Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F17%3A00077932" target="_blank" >RIV/00209805:_____/17:00077932 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.degruyter.com/view/j/revac.ahead-of-print/revac-2016-0022/revac-2016-0022.xml" target="_blank" >https://www.degruyter.com/view/j/revac.ahead-of-print/revac-2016-0022/revac-2016-0022.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/revac-2016-0022" target="_blank" >10.1515/revac-2016-0022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs

Popis výsledku v původním jazyce

Dysregulation of gene expression mechanisms has been observed in many tumors, making their analysis of utmost importance. These mechanisms include DNA methylation, an epigenetic mechanism in which 5-carbon of cytosine becomes methylated, leading to gene silencing, and action of short RNA molecules called microRNAs, which regulate protein synthesis at post-transcriptional level by binding to mRNAs. In this review, we describe major roles of both mechanisms in carcinogenesis, offer an overview of currently used methods for their analysis, and summarize most recent advances in electrochemicalbased assays and strategies. Advantages of electrochemistry, including favorable cost, time of experiment, or simple instrumentation, are highlighted, along with current challenges that need to be addressed prior to successful application into clinical routine.

Název v anglickém jazyce

Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs

Popis výsledku anglicky

Dysregulation of gene expression mechanisms has been observed in many tumors, making their analysis of utmost importance. These mechanisms include DNA methylation, an epigenetic mechanism in which 5-carbon of cytosine becomes methylated, leading to gene silencing, and action of short RNA molecules called microRNAs, which regulate protein synthesis at post-transcriptional level by binding to mRNAs. In this review, we describe major roles of both mechanisms in carcinogenesis, offer an overview of currently used methods for their analysis, and summarize most recent advances in electrochemicalbased assays and strategies. Advantages of electrochemistry, including favorable cost, time of experiment, or simple instrumentation, are highlighted, along with current challenges that need to be addressed prior to successful application into clinical routine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reviews in Analytical Chemistry

ISSN

0793-0135

e-ISSN

—

Svazek periodika

36

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

20160022

Kód UT WoS článku

000396218200002

EID výsledku v databázi Scopus

2-s2.0-85016086101