Intrinsic Electrocatalysis in DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F18%3A00077987" target="_blank" >RIV/00209805:_____/18:00077987 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/18:00506881

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/celc.201700888" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/celc.201700888</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/celc.201700888" target="_blank" >10.1002/celc.201700888</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Intrinsic Electrocatalysis in DNA

Popis výsledku v původním jazyce

We show, for the first time, that DNA produces an electrocatalytic square wave voltammetric reduction peak or chronopotentiometric peak HDNA at mercury and solid amalgam electrodes. These peaks are attributed to the catalytic hydrogen evolution reaction, allowing label-free detection of chromosomal DNA and oligodeoxynucleotides (ODNs) below parts per million and nanomolar levels, respectively. By using peak HDNA at high current densities, native chromosomal DNA was distinguished from its denatured form. With this method, damage of DNA from human cancer cells by ionizing radiation or sonication was detected. Sensitivity of the DNA determination was greatly enhanced in the presence of [Co(NH3)6]3+. Chromosomal DNA and ODNs can be detected at concentrations 2-3 orders lower than any earlier voltammetric or chronopotentiometric label-free methods. In the presence of 4 mM [Co(NH3)6]3+, DNA from human cancer cells was easily detected at 5 ng/mL, that is, below the concentration level of cell-free DNA in body fluids.

Název v anglickém jazyce

Intrinsic Electrocatalysis in DNA

Popis výsledku anglicky

We show, for the first time, that DNA produces an electrocatalytic square wave voltammetric reduction peak or chronopotentiometric peak HDNA at mercury and solid amalgam electrodes. These peaks are attributed to the catalytic hydrogen evolution reaction, allowing label-free detection of chromosomal DNA and oligodeoxynucleotides (ODNs) below parts per million and nanomolar levels, respectively. By using peak HDNA at high current densities, native chromosomal DNA was distinguished from its denatured form. With this method, damage of DNA from human cancer cells by ionizing radiation or sonication was detected. Sensitivity of the DNA determination was greatly enhanced in the presence of [Co(NH3)6]3+. Chromosomal DNA and ODNs can be detected at concentrations 2-3 orders lower than any earlier voltammetric or chronopotentiometric label-free methods. In the presence of 4 mM [Co(NH3)6]3+, DNA from human cancer cells was easily detected at 5 ng/mL, that is, below the concentration level of cell-free DNA in body fluids.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemElectroChem

ISSN

2196-0216

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

936-942

Kód UT WoS článku

000428374400014

EID výsledku v databázi Scopus

2-s2.0-85034867442