IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080254" target="_blank" >RIV/00209805:_____/25:00080254 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00141862

Výsledek na webu

<a href="https://www.spandidos-publications.com/or/53/6/71#" target="_blank" >https://www.spandidos-publications.com/or/53/6/71#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2025.8904" target="_blank" >10.3892/or.2025.8904</a>

Jazyk výsledku

angličtina

Název v původním jazyce

IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells

Popis výsledku v původním jazyce

Interferon-induced transmembrane proteins (IFITMs) are frequently overexpressed in cancer cells, including cervical carcinoma cells, and play a role in the progression of various cancer types. However, their mechanisms of action remain incompletely understood. In the present study, by employing a combination of surface membrane protein isolation and quantitative mass spectrometry, it was comprehensively described how the IFITM1 protein influences the composition of the cervical cancer cell surfaceome. Additionally, the effects of interferon-γ on protein expression and cell surface exposure were evaluated in the presence and absence of IFITM1. The IFITM1-regulated membrane and membrane-associated proteins identified are involved mainly in processes such as endocytosis and lysosomal transport, cell-cell and cell-extracellular matrix adhesion, antigen presentation and the immune response. To complement the proteomic data, gene expression was analyzed using reverse transcription-quantitative PCR to distinguish whether the observed changes in protein levels were attributable to transcriptional regulation or differential protein dynamics. Furthermore, the proteomic and gene expression data are supported by functional studies demonstrating the impact of the IFITM1 and IFITM3 proteins on the adhesive, migratory and invasive capabilities of cervical cancer cells, as well as their interactions with immune cells.

Název v anglickém jazyce

IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells

Popis výsledku anglicky

Interferon-induced transmembrane proteins (IFITMs) are frequently overexpressed in cancer cells, including cervical carcinoma cells, and play a role in the progression of various cancer types. However, their mechanisms of action remain incompletely understood. In the present study, by employing a combination of surface membrane protein isolation and quantitative mass spectrometry, it was comprehensively described how the IFITM1 protein influences the composition of the cervical cancer cell surfaceome. Additionally, the effects of interferon-γ on protein expression and cell surface exposure were evaluated in the presence and absence of IFITM1. The IFITM1-regulated membrane and membrane-associated proteins identified are involved mainly in processes such as endocytosis and lysosomal transport, cell-cell and cell-extracellular matrix adhesion, antigen presentation and the immune response. To complement the proteomic data, gene expression was analyzed using reverse transcription-quantitative PCR to distinguish whether the observed changes in protein levels were attributable to transcriptional regulation or differential protein dynamics. Furthermore, the proteomic and gene expression data are supported by functional studies demonstrating the impact of the IFITM1 and IFITM3 proteins on the adhesive, migratory and invasive capabilities of cervical cancer cells, as well as their interactions with immune cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Oncology reports

ISSN

1021-335X

e-ISSN

1791-2431

Svazek periodika

53

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

16

Strana od-do

71

Kód UT WoS článku

001484857100001

EID výsledku v databázi Scopus

2-s2.0-105003946572