Cytokinin arabinosides show increased efficacy in priming tomato immunity and inhibiting the fungal phytopathogen B. cinerea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080443" target="_blank" >RIV/00209805:_____/25:00080443 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/25:00641187 RIV/61989592:15310/25:73632016

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2667064X25003239" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2667064X25003239</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.stress.2025.101055" target="_blank" >10.1016/j.stress.2025.101055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytokinin arabinosides show increased efficacy in priming tomato immunity and inhibiting the fungal phytopathogen B. cinerea

Popis výsledku v původním jazyce

Cytokinins (CKs) regulate numerous biological processes in plants, and are involved in plant growth and development as well as responses to biotic and abiotic stresses. Although CKs have been reported to prime plant immunity, thus far, research on CK hormopriming has focused on classical synthetic CKs such as kinetin or 6-benzylaminopurine (BAP). Here, we show that synthetic aromatic CK derivatives-specifically, 3-methoxy-6-benzylaminopurine-9-arabinosides-effectively induce immune responses and promote resistance to Botrytis cinerea in tomato (Solanum lycopersicum) primarily via a salicylic acid (SA)-dependent mechanism. Additionally, like 6- BAP, these compounds directly inhibit B. cinerea. Transcriptomic analyses of B. cinerea treated with CK arabinosides revealed significant changes in expressed gene profiles, notably in pathways associated with sugar metabolism. Unlike classical CKs, CK arabinosides do not alleviate growth inhibition under sugar-limited conditions, contributing to their stronger antifungal effects. Furthermore, CK arabinosides induce tomato immune responses more robustly than classical CKs, yet without activating canonical cytokinin signaling pathways, likely owing to their exceptional chemical stability which prevents release of the free active CK base, and due to their inability to directly bind CK receptors. Altogether, our findings suggest that CK arabinosides act via a dual mechanism-immune priming and direct pathogen inhibition-and hold promise as next-generation hormopriming agents for sustainable crop protection.

Název v anglickém jazyce

Cytokinin arabinosides show increased efficacy in priming tomato immunity and inhibiting the fungal phytopathogen B. cinerea

Popis výsledku anglicky

Cytokinins (CKs) regulate numerous biological processes in plants, and are involved in plant growth and development as well as responses to biotic and abiotic stresses. Although CKs have been reported to prime plant immunity, thus far, research on CK hormopriming has focused on classical synthetic CKs such as kinetin or 6-benzylaminopurine (BAP). Here, we show that synthetic aromatic CK derivatives-specifically, 3-methoxy-6-benzylaminopurine-9-arabinosides-effectively induce immune responses and promote resistance to Botrytis cinerea in tomato (Solanum lycopersicum) primarily via a salicylic acid (SA)-dependent mechanism. Additionally, like 6- BAP, these compounds directly inhibit B. cinerea. Transcriptomic analyses of B. cinerea treated with CK arabinosides revealed significant changes in expressed gene profiles, notably in pathways associated with sugar metabolism. Unlike classical CKs, CK arabinosides do not alleviate growth inhibition under sugar-limited conditions, contributing to their stronger antifungal effects. Furthermore, CK arabinosides induce tomato immune responses more robustly than classical CKs, yet without activating canonical cytokinin signaling pathways, likely owing to their exceptional chemical stability which prevents release of the free active CK base, and due to their inability to directly bind CK receptors. Altogether, our findings suggest that CK arabinosides act via a dual mechanism-immune priming and direct pathogen inhibition-and hold promise as next-generation hormopriming agents for sustainable crop protection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: Nové poznatky pro plodiny nové generace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant stress

ISSN

2667-064X

e-ISSN

2667-064X

Svazek periodika

18

Číslo periodika v rámci svazku

December 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

101055

Kód UT WoS článku

001594551300001

EID výsledku v databázi Scopus

2-s2.0-105018178878