Circulating Serum piRNAs as Diagnostic and Prognostic Tools for Clear Cell Renal Cell Carcinoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080450" target="_blank" >RIV/00209805:_____/25:00080450 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/25:00142393 RIV/65269705:_____/25:00082937

Výsledek na webu

<a href="https://cgp.iiarjournals.org/content/22/6/888.long" target="_blank" >https://cgp.iiarjournals.org/content/22/6/888.long</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21873/cgp.20545" target="_blank" >10.21873/cgp.20545</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Circulating Serum piRNAs as Diagnostic and Prognostic Tools for Clear Cell Renal Cell Carcinoma

Popis výsledku v původním jazyce

BACKGROUND/AIM: The incidence of cancer continues to rise, highlighting the urgent need for reliable, non-invasive biomarkers to support early diagnosis and improve treatment outcomes. In addition to circulating microRNAs, circulating PIWI-interacting RNAs (cir-piRNAs) have emerged as promising candidates. Although the biological functions of piRNAs are not yet fully understood, they are known to suppress transposable elements and may regulate structural genes involved in tumorigenesis. In this two-phase study, we aimed to identify serum piRNAs with diagnostic potential for clear cell renal cell carcinoma (ccRCC). MATERIALS AND METHODS: A total of 238 serum samples from ccRCC patients and 208 healthy controls were analyzed. In the exploratory phase, next-generation sequencing (NGS) was performed on pooled samples representing different clinical stages of ccRCC and healthy controls. RESULTS: We identified 35 piRNAs with significantly different expression (p&lt;0.01) between groups. Based on statistical significance, read abundance, and fold change, six piRNAs were selected for the training phase and subsequently, three piRNAs, piR-24672, piR-27140, and piR-28876, were selected for validation. Validation by RT-qPCR in an independent cohort confirmed significantly reduced levels of all three piRNAs in ccRCC patients. ROC analysis demonstrated that piR-28876 is a superior diagnostic biomarker, reaching AUC=0.787, with 85.0% specificity and 66.3% sensitivity in distinguishing ccRCC patients from healthy controls. CONCLUSION: Circulating piRNAs, particularly piR-24672, piR-27140, and piR-28876, may serve as potential non-invasive biomarkers for the early detection of ccRCC.

Název v anglickém jazyce

Circulating Serum piRNAs as Diagnostic and Prognostic Tools for Clear Cell Renal Cell Carcinoma

Popis výsledku anglicky

BACKGROUND/AIM: The incidence of cancer continues to rise, highlighting the urgent need for reliable, non-invasive biomarkers to support early diagnosis and improve treatment outcomes. In addition to circulating microRNAs, circulating PIWI-interacting RNAs (cir-piRNAs) have emerged as promising candidates. Although the biological functions of piRNAs are not yet fully understood, they are known to suppress transposable elements and may regulate structural genes involved in tumorigenesis. In this two-phase study, we aimed to identify serum piRNAs with diagnostic potential for clear cell renal cell carcinoma (ccRCC). MATERIALS AND METHODS: A total of 238 serum samples from ccRCC patients and 208 healthy controls were analyzed. In the exploratory phase, next-generation sequencing (NGS) was performed on pooled samples representing different clinical stages of ccRCC and healthy controls. RESULTS: We identified 35 piRNAs with significantly different expression (p&lt;0.01) between groups. Based on statistical significance, read abundance, and fold change, six piRNAs were selected for the training phase and subsequently, three piRNAs, piR-24672, piR-27140, and piR-28876, were selected for validation. Validation by RT-qPCR in an independent cohort confirmed significantly reduced levels of all three piRNAs in ccRCC patients. ROC analysis demonstrated that piR-28876 is a superior diagnostic biomarker, reaching AUC=0.787, with 85.0% specificity and 66.3% sensitivity in distinguishing ccRCC patients from healthy controls. CONCLUSION: Circulating piRNAs, particularly piR-24672, piR-27140, and piR-28876, may serve as potential non-invasive biomarkers for the early detection of ccRCC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cancer genomics &amp; proteomics

ISSN

1109-6535

e-ISSN

1790-6245

Svazek periodika

22

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

12

Strana od-do

888-899

Kód UT WoS článku

001607415600005

EID výsledku v databázi Scopus

2-s2.0-105020356889