Diversity among wild type and vaccination strains of Trichophyton verrucosum investigated using Random Amplified Polymotphic DNA Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F99%3A00000064" target="_blank" >RIV/00209805:_____/99:00000064 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity among wild type and vaccination strains of Trichophyton verrucosum investigated using Random Amplified Polymotphic DNA Analysis

Popis výsledku v původním jazyce

We initially tested 20 primers for their ability to amplify genomic DNA of Trichophyton verrucosum using RAPD. Six of these were selected for further study aimed at discrimination of wild type and vaccination strains of T. verrucosum. The results indicate that RAPD successfully distinguished all strains included in the study. In addition, results of corrected cluster analysis were consistent with the fact that the avirulent vaccination strains (T. verrucosum TV-M9 and T. verr (T. verrucosum TV-M9 and T.verrucosum TV-M-130) were prepared by ultraviolet (UV) light induced mutagenesis of the standard wild type strain T. verrucosum Strážnice. No marker for a/virulence was detected. These outcomes suggest new possibilities for epidemiological analyses, fordiscrimination among different vaccination strains and studies of fungal population in vaccinated/infected hosts.

Název v anglickém jazyce

Diversity among wild type and vaccination strains of Trichophyton verrucosum investigated using Random Amplified Polymotphic DNA Analysis

Popis výsledku anglicky

We initially tested 20 primers for their ability to amplify genomic DNA of Trichophyton verrucosum using RAPD. Six of these were selected for further study aimed at discrimination of wild type and vaccination strains of T. verrucosum. The results indicate that RAPD successfully distinguished all strains included in the study. In addition, results of corrected cluster analysis were consistent with the fact that the avirulent vaccination strains (T. verrucosum TV-M9 and T. verr (T. verrucosum TV-M9 and T.verrucosum TV-M-130) were prepared by ultraviolet (UV) light induced mutagenesis of the standard wild type strain T. verrucosum Strážnice. No marker for a/virulence was detected. These outcomes suggest new possibilities for epidemiological analyses, fordiscrimination among different vaccination strains and studies of fungal population in vaccinated/infected hosts.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Biologica

ISSN

0015-5497

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

neuv.

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—