Cytokeratins 8 and 18 in adult human corneal endothelium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F09%3A3500" target="_blank" >RIV/00216208:11110/09:3500 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/09:00001715 RIV/00064165:_____/09:3500

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytokeratins 8 and 18 in adult human corneal endothelium

Popis výsledku v původním jazyce

The aim of this study was to determine if cytokeratins (CKs) 8 and 18 - typical epithelial cell markers - are expressed in human corneal endothelium. Cryosections, paraffin-embedded sections and corneal endothelial imprints obtained from eleven corneal discs were used. Different fixative solutions were applied before indirect immunofluorescent or enzymatic staining. Antibodies against CK8, CK18 and CK8/18 were used and semi-quantitative RT-PCR and Western blotting were performed. Approximately 50% of the corneal endothelial cells were positive for CK8, CK18 and the CK8/18 after acetone fixation. No signal was detected when 4% formalin or 10% paraformaldehyde was used. CK8 and CK18 were detected in the endothelium of all tested corneas by Western blotting or semi-quantitative RT-PCR, respectively. We detected CK8 and CK18 in the endothelium of all specimens. These results clearly demonstrate that cells of the corneal endothelium express CKs 8 and 18 and share some features with simple e

Název v anglickém jazyce

Cytokeratins 8 and 18 in adult human corneal endothelium

Popis výsledku anglicky

The aim of this study was to determine if cytokeratins (CKs) 8 and 18 - typical epithelial cell markers - are expressed in human corneal endothelium. Cryosections, paraffin-embedded sections and corneal endothelial imprints obtained from eleven corneal discs were used. Different fixative solutions were applied before indirect immunofluorescent or enzymatic staining. Antibodies against CK8, CK18 and CK8/18 were used and semi-quantitative RT-PCR and Western blotting were performed. Approximately 50% of the corneal endothelial cells were positive for CK8, CK18 and the CK8/18 after acetone fixation. No signal was detected when 4% formalin or 10% paraformaldehyde was used. CK8 and CK18 were detected in the endothelium of all tested corneas by Western blotting or semi-quantitative RT-PCR, respectively. We detected CK8 and CK18 in the endothelium of all specimens. These results clearly demonstrate that cells of the corneal endothelium express CKs 8 and 18 and share some features with simple e

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FF - ORL, oftalmologie, stomatologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Experimental Eye Research

ISSN

0014-4835

e-ISSN

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Svazek periodika

89

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000269041900016

EID výsledku v databázi Scopus

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