Mitochondrial DNA content and expression of genes involved in mtDNA transcription, regulation and maintenance during human fetal development
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F10%3A6825" target="_blank" >RIV/00216208:11110/10:6825 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00064165:_____/10:6825
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Mitochondrial DNA content and expression of genes involved in mtDNA transcription, regulation and maintenance during human fetal development
Popis výsledku v původním jazyce
The mitochondrial biogenesis and adequate energy production are important for fetal growth and early postnatal adaptation. The aim of the study was to characterize mitochondrial DNA (mtDNA) content and expression patterns of POLG, TFAM, NRF1,NRF2 and PGC1 family of regulated coactivators (PGC1A, PGC1B and PRC), COX4 and MTCO2 in human fetal tissues during gestation. Moreover enzyme activities of COX and CS and protein levels of cytochrome c oxidase (COX) subunits were analysed. DNA, RNA and proteins were isolated from 26 pairs of fetal liver and muscle samples between 13th and 28th week of gestation. This work offers a broad view on the differences in expression of POLG, TFAM, NRF2 genes and family PGC1 coactivators found between the fetal tissues. Thesignificant tissue-specific changes in expression of selected COX subunits on mRNA level were observed. The considerable differences in enzyme activities of COX and CS are demonstrated between fetal and postnatal phase. In conclusion our
Název v anglickém jazyce
Mitochondrial DNA content and expression of genes involved in mtDNA transcription, regulation and maintenance during human fetal development
Popis výsledku anglicky
The mitochondrial biogenesis and adequate energy production are important for fetal growth and early postnatal adaptation. The aim of the study was to characterize mitochondrial DNA (mtDNA) content and expression patterns of POLG, TFAM, NRF1,NRF2 and PGC1 family of regulated coactivators (PGC1A, PGC1B and PRC), COX4 and MTCO2 in human fetal tissues during gestation. Moreover enzyme activities of COX and CS and protein levels of cytochrome c oxidase (COX) subunits were analysed. DNA, RNA and proteins were isolated from 26 pairs of fetal liver and muscle samples between 13th and 28th week of gestation. This work offers a broad view on the differences in expression of POLG, TFAM, NRF2 genes and family PGC1 coactivators found between the fetal tissues. Thesignificant tissue-specific changes in expression of selected COX subunits on mRNA level were observed. The considerable differences in enzyme activities of COX and CS are demonstrated between fetal and postnatal phase. In conclusion our
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Mitochondrion
ISSN
1567-7249
e-ISSN
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Svazek periodika
10
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
9
Strana od-do
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Kód UT WoS článku
000278262600003
EID výsledku v databázi Scopus
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