Fine structure of the "PcG body" in human U-2 OS cells established by correlative light-electron microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10482" target="_blank" >RIV/00216208:11110/11:10482 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4161/nucl.2.3.15737" target="_blank" >http://dx.doi.org/10.4161/nucl.2.3.15737</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fine structure of the "PcG body" in human U-2 OS cells established by correlative light-electron microscopy

Popis výsledku v původním jazyce

Polycomb group (PcG) proteins of the Polycomb repressive complex 1 (PRC1) are found to be diffusely distributed in nuclei of cells from various species. However they can also be localized in intensely fluorescent foci, whether imaged using GFP fusions toproteins of PRC1 complex, or by conventional immunofluorescence microscopy. Such foci are termed Polycomb bodies (PcG bodies), and are believed to be situated into the nuclear intechromatin compartment. However, an ultrastructural description of the PcGbody has not been reported to date. To establish the ultrastructure of PcG bodies in human U-2 OS cells stably expressing recombinant polycomb BMI1-GFP protein, we used correlative light electron microscopy (CLEM) implemented with high-pressure freezing, cryosubstitution and on section labeling of BMI1 protein with immunogold. This approach allowed us to clearly identify fluorescent PcG bodies, not as distinct nuclear bodies, but as nuclear domains enriched in separated heterochromatin

Název v anglickém jazyce

Fine structure of the "PcG body" in human U-2 OS cells established by correlative light-electron microscopy

Popis výsledku anglicky

Polycomb group (PcG) proteins of the Polycomb repressive complex 1 (PRC1) are found to be diffusely distributed in nuclei of cells from various species. However they can also be localized in intensely fluorescent foci, whether imaged using GFP fusions toproteins of PRC1 complex, or by conventional immunofluorescence microscopy. Such foci are termed Polycomb bodies (PcG bodies), and are believed to be situated into the nuclear intechromatin compartment. However, an ultrastructural description of the PcGbody has not been reported to date. To establish the ultrastructure of PcG bodies in human U-2 OS cells stably expressing recombinant polycomb BMI1-GFP protein, we used correlative light electron microscopy (CLEM) implemented with high-pressure freezing, cryosubstitution and on section labeling of BMI1 protein with immunogold. This approach allowed us to clearly identify fluorescent PcG bodies, not as distinct nuclear bodies, but as nuclear domains enriched in separated heterochromatin

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC535" target="_blank" >LC535: Dynamika a organizace chromosomů během buněčného cyklu v normě a patologii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nucleus

ISSN

1949-1034

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

219-228

Kód UT WoS článku

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EID výsledku v databázi Scopus

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